Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator

Tania Chroumpi; Maria Victoria Aguilar-Pontes; Mao Peng; Mei Wang; Anna Lipzen; Vivian Ng; Igor V. Grigoriev; Miia R. Mäkelä; Ronald P. de Vries

doi:10.1016/j.micres.2020.126426

Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator

Tania Chroumpi, Maria Victoria Aguilar-Pontes, Mao Peng, Mei Wang, Anna Lipzen, Vivian Ng, Igor V. Grigoriev, Miia R. Mäkelä, Ronald P. de Vries^*

^*Corresponding author for this work

Molecular Plant Physiology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.

Original language	English
Article number	126426
Pages (from-to)	1-10
Number of pages	10
Journal	Microbiological Research
Volume	234
DOIs	https://doi.org/10.1016/j.micres.2020.126426
Publication status	Published - Apr 2020

Funding

TC was supported by a grant of the NWO ALWOP.233 to RPdV. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Academy of Finland grant no. 308284 to MRM is acknowledged.

Keywords

Gene regulation
Inducer
L-rhamnose catabolic pathway
Pectinolytic enzymes
RhaR

Access to Document

10.1016/j.micres.2020.126426

1-s2.0-S0944501319312790-mainFinal published version, 3.6 MBLicence: Taverne

Cite this

Chroumpi, T., Aguilar-Pontes, M. V., Peng, M., Wang, M., Lipzen, A., Ng, V., Grigoriev, I. V., Mäkelä, M. R., & de Vries, R. P. (2020). Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator. Microbiological Research, 234, 1-10. Article 126426. https://doi.org/10.1016/j.micres.2020.126426

@article{de08b765c34a4a07be4b76d3150f3c03,

title = "Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator",

abstract = "In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.",

keywords = "Gene regulation, Inducer, L-rhamnose catabolic pathway, Pectinolytic enzymes, RhaR",

author = "Tania Chroumpi and Aguilar-Pontes, {Maria Victoria} and Mao Peng and Mei Wang and Anna Lipzen and Vivian Ng and Grigoriev, {Igor V.} and M{\"a}kel{\"a}, {Miia R.} and {de Vries}, {Ronald P.}",

year = "2020",

month = apr,

doi = "10.1016/j.micres.2020.126426",

language = "English",

volume = "234",

pages = "1--10",

journal = "Microbiological Research",

issn = "0944-5013",

publisher = "Elsevier GmbH",

}

Chroumpi, T, Aguilar-Pontes, MV, Peng, M, Wang, M, Lipzen, A, Ng, V, Grigoriev, IV, Mäkelä, MR & de Vries, RP 2020, 'Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator', Microbiological Research, vol. 234, 126426, pp. 1-10. https://doi.org/10.1016/j.micres.2020.126426

TY - JOUR

T1 - Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator

AU - Chroumpi, Tania

AU - Aguilar-Pontes, Maria Victoria

AU - Peng, Mao

AU - Wang, Mei

AU - Lipzen, Anna

AU - Ng, Vivian

AU - Grigoriev, Igor V.

AU - Mäkelä, Miia R.

AU - de Vries, Ronald P.

PY - 2020/4

Y1 - 2020/4

N2 - In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.

AB - In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.

KW - Gene regulation

KW - Inducer

KW - L-rhamnose catabolic pathway

KW - Pectinolytic enzymes

KW - RhaR

UR - http://www.scopus.com/inward/record.url?scp=85079130185&partnerID=8YFLogxK

U2 - 10.1016/j.micres.2020.126426

DO - 10.1016/j.micres.2020.126426

M3 - Article

AN - SCOPUS:85079130185

SN - 0944-5013

VL - 234

SP - 1

EP - 10

JO - Microbiological Research

JF - Microbiological Research

M1 - 126426

ER -

Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this