Identification and characterization of two novel viruses in ocular infections in reindeer

Saskia L Smits; Claudia M E Schapendonk; Marije van Leeuwen; Thijs Kuiken; Rogier Bodewes; V Stalin Raj; Bart L Haagmans; Carlos G das Neves; Morten Tryland; Albert D M E Osterhaus

doi:10.1371/journal.pone.0069711

Identification and characterization of two novel viruses in ocular infections in reindeer

Saskia L Smits, Claudia M E Schapendonk, Marije van Leeuwen, Thijs Kuiken, Rogier Bodewes, V Stalin Raj, Bart L Haagmans, Carlos G das Neves, Morten Tryland, Albert D M E Osterhaus

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based on host nucleic acid depletion, sequence-independent amplification and next-generation sequencing of partially purified viral nucleic acid, revealed the presence of a new papillomavirus in 2 out of 8 eye swab samples and a new betaherpesvirus in 3 out of 8 eye swab samples collected from animals with clinical signs and not in similar samples in 9 animals without clinical signs. Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.

Original language	English
Pages (from-to)	e69711
Journal	PLoS One
Volume	8
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0069711
Publication status	Published - 2013

Keywords

Animals
Eye Infections
Papillomaviridae
Phylogeny
Reindeer

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title = "Identification and characterization of two novel viruses in ocular infections in reindeer",

abstract = "A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based on host nucleic acid depletion, sequence-independent amplification and next-generation sequencing of partially purified viral nucleic acid, revealed the presence of a new papillomavirus in 2 out of 8 eye swab samples and a new betaherpesvirus in 3 out of 8 eye swab samples collected from animals with clinical signs and not in similar samples in 9 animals without clinical signs. Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.",

keywords = "Animals, Eye Infections, Papillomaviridae, Phylogeny, Reindeer",

author = "Smits, {Saskia L} and Schapendonk, {Claudia M E} and {van Leeuwen}, Marije and Thijs Kuiken and Rogier Bodewes and {Stalin Raj}, V and Haagmans, {Bart L} and {das Neves}, {Carlos G} and Morten Tryland and Osterhaus, {Albert D M E}",

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AU - Smits, Saskia L

AU - Schapendonk, Claudia M E

AU - van Leeuwen, Marije

AU - Kuiken, Thijs

AU - Bodewes, Rogier

AU - Stalin Raj, V

AU - Haagmans, Bart L

AU - das Neves, Carlos G

AU - Tryland, Morten

AU - Osterhaus, Albert D M E

PY - 2013

Y1 - 2013

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AB - A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based on host nucleic acid depletion, sequence-independent amplification and next-generation sequencing of partially purified viral nucleic acid, revealed the presence of a new papillomavirus in 2 out of 8 eye swab samples and a new betaherpesvirus in 3 out of 8 eye swab samples collected from animals with clinical signs and not in similar samples in 9 animals without clinical signs. Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.

KW - Animals

KW - Eye Infections

KW - Papillomaviridae

KW - Phylogeny

KW - Reindeer

U2 - 10.1371/journal.pone.0069711

DO - 10.1371/journal.pone.0069711

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SP - e69711

JO - PLoS One

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IS - 7

ER -

Identification and characterization of two novel viruses in ocular infections in reindeer

Abstract

Keywords

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