Hypotensive effect of 13-hydroxylinoleic acid in the rat: Mediation via the release of a CGRP-like mediator from capsaicin-sensitive nerves

1. The effect of 13-hydroxylinoleic acid (13-HODE) on changes in blood pressure in the rat was measured. 2. 13-HODE (0.1-100 μg kg-1) had no direct effect on blood pressure in the rat and had no effect on histamine (0.1-1000 μg kg-1-induced changes in blood pressure. In contrast, it was found that 13-HODE itself induced a decrease in diastolic arterial blood pressure when it was injected intravenously after either a single dose of histamine (10, 100 or 1000 μg kg-1) or after a dose-response curve of histamine (0.1-1000 μg kg-1). 3. This hypotensive effect of 13-HODE was not observed after administration of the endothelium-dependent vasodilator, acetylcholine (0.1-10 μg kg-1), the endothelium-independent vasodilator, sodium nitroprusside (0.1-100 μg kg-1) or the inflammatory mediator, leukotriene B4(0.1-300 μg kg-1). However, prior injection of bradykinin (0.1-100 μg kg-1) allowed a dose-dependent hypotensive effect of 13-HODE to be revealed. 4. The hypotensive effect of 13-HODE after histamine and bradykinin could be inhibited by neonatal capsaicin treatment of the rats (50 mg kg-1, s.c. on day 1 and 2 after birth). 5. Ruthenium red (120 μg kg-1min-1), an inhibitor of excitatory effects on sensory nerves, and the CGRP antagonist, CGRP8-37(1-3 μg kg-1min-1) also inhibited the hypotensive effect of 13-HODE. 6. It is concluded that the hypotensive effect of 13-HODE in the rat after histamine and bradykinin is due to the release of a CGRP-like substance from sensory nerves. These results highlight the possibility that endogenous 13-HODE could be involved in the neurogenic regulation of blood pressure.