Hydantoin: The mechanism of its in vitro anti-enterovirus activity revisited

Aloys Tijsma, Hendrik Jan Thibaut, David Franco, Kai Dallmeier, Johan Neyts

Research output: Contribution to journalArticleAcademicpeer-review


It has been generally accepted that hydantoin [5-(3,4-dichlorophenyl)methylhydantoin] exerts its anti-enterovirus activity by solely inhibiting viral assembly. However, we here show that hydantoin inhibits enteroviral RNA synthesis as well as subgenomic replication in a dose-dependent manner. We demonstrate that inhibition of RNA synthesis is the predominant mechanism of action at relatively high concentrations of hydantoin. However, at lower concentrations inhibition of viral morphogenesis is the main mechanism of action. Thus, hydantoin inhibits enteroviral replication by two distinct mechanisms.

Original languageEnglish
Pages (from-to)106-109
Number of pages4
JournalAntiviral Research
Publication statusPublished - Sept 2016
Externally publishedYes


  • Hydantoin
  • Enterovirus
  • Morphogenesis
  • RNA replication


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