TY - JOUR
T1 - Human Wharton's jelly mesenchymal stem cells protect neural cells from oxidative stress through paracrine mechanisms
AU - Puig-Pijuan, Teresa
AU - de Godoy, Mariana A
AU - Pinheiro Carvalho, Luiza Rachel
AU - Bodart-Santos, Victor
AU - Lindoso, Rafael Soares
AU - Pimentel-Coelho, Pedro Moreno
AU - Mendez-Otero, Rosalia
N1 - © 2020 Teresa Puig-Pijuan and Rosalia Mendez-Otero.
PY - 2020/9/17
Y1 - 2020/9/17
N2 - Aim: Mesenchymal stem cells (MSCs) have neuroprotective and immunomodulatory properties, which are partly mediated by extracellular vesicles (EVs) secretion. We aimed to evaluate the effects of human Wharton's jelly-derived MSCs (WJ-MSCs) and their EVs on rat hippocampal cultures subjected to hydrogen peroxide (H2O2).Materials & methods: Hippocampal dissociated cultures were either co-cultured with WJ-MSCs or treated with their EVs prior to H2O2 exposure and reactive oxygen species levels and cell viability were evaluated.Results: Coculture with WJ-MSCs or pre-incubation with EVs prior to the insult reduced reactive oxygen species after H2O2 exposure. Cell viability was improved only when coculture was maintained following the insult, while EVs did not significantly improve cell viability.Conclusion: WJ-MSCs have potential antioxidant and neuroprotective effects on hippocampal cultures which might be partially mediated by EVs.
AB - Aim: Mesenchymal stem cells (MSCs) have neuroprotective and immunomodulatory properties, which are partly mediated by extracellular vesicles (EVs) secretion. We aimed to evaluate the effects of human Wharton's jelly-derived MSCs (WJ-MSCs) and their EVs on rat hippocampal cultures subjected to hydrogen peroxide (H2O2).Materials & methods: Hippocampal dissociated cultures were either co-cultured with WJ-MSCs or treated with their EVs prior to H2O2 exposure and reactive oxygen species levels and cell viability were evaluated.Results: Coculture with WJ-MSCs or pre-incubation with EVs prior to the insult reduced reactive oxygen species after H2O2 exposure. Cell viability was improved only when coculture was maintained following the insult, while EVs did not significantly improve cell viability.Conclusion: WJ-MSCs have potential antioxidant and neuroprotective effects on hippocampal cultures which might be partially mediated by EVs.
KW - cell therapy
KW - extracellular vesicles
KW - medicinal signaling cells
KW - mesenchymal stem cells
KW - oxidative stress
KW - paracrine signaling
KW - umbilical cord Wharton’s jelly
U2 - 10.2144/fsoa-2020-0036
DO - 10.2144/fsoa-2020-0036
M3 - Article
C2 - 33235812
SN - 2056-5623
VL - 6
JO - Future Cardiology
JF - Future Cardiology
IS - 9
M1 - FSO627
ER -