TY - JOUR
T1 - Human milk oligosaccharides affect P-selectin binding capacities
T2 - In vitro investigation
AU - Schumacher, Gabriele
AU - Bendas, Gerd
AU - Stahl, Bernd
AU - Beermann, Christopher
PY - 2006/6
Y1 - 2006/6
N2 - Objective: In the initial phase of cellular immune response, selectins mediate the emigration of leukocytes from the blood stream into inflammatory regions. Human milk oligosaccharides (HMOs) possess binding epitopes of selectin ligands such as sialyl Lewisx and sialyl Lewisa and therefore might impair the interaction of selectins with cellular ligands. Neutral, acidic, sialylated, or fucosylated HMO fractions with polymerization degrees of 3 to 50 were investigated regarding this interaction in a dynamic flow chamber model that considers physiologic shear stress conditions. Methods: Human milk oligosaccharides were compared with κ-carrageenans and pectin oligosaccharides to deduce structure-activity relations. Fucoidan and sialyl Lewisx served as positive controls. Results: All HMO fractions affected P-selectin ligand binding capacity but were not comparable to fucoidan. The activity of the acidic HMO fraction resembled sialyl Lewisx in decreasing the binding of the ligand to P-selectin. Conclusion: Human milk oligosaccharides modulate rather than block the function of P-selectin.
AB - Objective: In the initial phase of cellular immune response, selectins mediate the emigration of leukocytes from the blood stream into inflammatory regions. Human milk oligosaccharides (HMOs) possess binding epitopes of selectin ligands such as sialyl Lewisx and sialyl Lewisa and therefore might impair the interaction of selectins with cellular ligands. Neutral, acidic, sialylated, or fucosylated HMO fractions with polymerization degrees of 3 to 50 were investigated regarding this interaction in a dynamic flow chamber model that considers physiologic shear stress conditions. Methods: Human milk oligosaccharides were compared with κ-carrageenans and pectin oligosaccharides to deduce structure-activity relations. Fucoidan and sialyl Lewisx served as positive controls. Results: All HMO fractions affected P-selectin ligand binding capacity but were not comparable to fucoidan. The activity of the acidic HMO fraction resembled sialyl Lewisx in decreasing the binding of the ligand to P-selectin. Conclusion: Human milk oligosaccharides modulate rather than block the function of P-selectin.
KW - Cell adhesion
KW - Human milk
KW - Inhibition
KW - Lectin
KW - Oligosaccharides
KW - Selectin
UR - http://www.scopus.com/inward/record.url?scp=33646509628&partnerID=8YFLogxK
U2 - 10.1016/j.nut.2005.12.009
DO - 10.1016/j.nut.2005.12.009
M3 - Article
C2 - 16533594
AN - SCOPUS:33646509628
SN - 0899-9007
VL - 22
SP - 620
EP - 627
JO - Nutrition
JF - Nutrition
IS - 6
ER -