Human IgE does not bind to human FcRn.

M Brinkhaus; der Kooi EJ van; AEH Bentlage; Heer P Ooijevaar-de; NIL Derksen; T Rispens; G Vidarsson

doi:10.1038/s41598-021-03852-1

Human IgE does not bind to human FcRn.

M Brinkhaus, der Kooi EJ van, AEH Bentlage, Heer P Ooijevaar-de, NIL Derksen, T Rispens, G Vidarsson

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.

Original language	English
Article number	62
Journal	Scientific Reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-03852-1
Publication status	Published - Dec 2022
Externally published	Yes

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title = "Human IgE does not bind to human FcRn.",

abstract = "The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.",

author = "M Brinkhaus and van, {der Kooi EJ} and AEH Bentlage and Ooijevaar-de, {Heer P} and NIL Derksen and T Rispens and G Vidarsson",

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doi = "10.1038/s41598-021-03852-1",

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AU - Brinkhaus, M

AU - van, der Kooi EJ

AU - Bentlage, AEH

AU - Ooijevaar-de, Heer P

AU - Derksen, NIL

AU - Rispens, T

AU - Vidarsson, G

PY - 2022/12

Y1 - 2022/12

N2 - The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.

AB - The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.

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DO - 10.1038/s41598-021-03852-1

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SN - 2045-2322

VL - 12

JO - Scientific Reports

JF - Scientific Reports

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Human IgE does not bind to human FcRn.

Abstract

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