HPD-induced photodynamic changes in intracellular cyclic AMP levels in human bladder transitional carcinoma cells, clone T24

L C Penning, J VanSteveninck, T M Dubbelman

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Human bladder transitional carcinoma cells, clone T24, were incubated with hematoporphyrin derivative and subsequently exposed to red light. Immediately after light exposure a light-dose dependent increase in intracellular cyclic AMP levels was observed. The concentrations decreased to non-treated levels within 2 h after illumination. Inhibition of cyclooxygenase with indomethacin reduced the increase drastically, whereas the presence of PGE2 enhanced the production of cyclic AMP. PGE2, 8'-bromo-cyclic AMP and a direct stimulation of adenylyl cyclase by forskolin reduced the photodynamically induced cell killing. On the other hand, suppression of cyclic AMP by the cyclooxygenase inhibitor indomethacin, enhanced the photodynamically induced cell killing. Therefore, it is suggested that cyclic AMP production in T24 cells is a step in a cellular rescue pathway, resulting in protection against photodynamic cell killing.

    Original languageEnglish
    Pages (from-to)1084-9
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume194
    Issue number3
    DOIs
    Publication statusPublished - 16 Aug 1993

    Keywords

    • 8-Bromo Cyclic Adenosine Monophosphate
    • Carcinoma, Transitional Cell
    • Cell Death
    • Cyclic AMP
    • Cyclooxygenase Inhibitors
    • Dinoprostone
    • Hematoporphyrin Derivative
    • Humans
    • Indomethacin
    • Light
    • Tumor Cells, Cultured
    • Urinary Bladder Neoplasms

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