How to screen non-viral gene delivery systems in vitro?

E.V.B. van Gaal, R. van Eijk, R.S. Oosting, R.J. Kok, W.E. Hennink, D.J.A. Crommelin, E. Mastrobattista

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field.
Original languageUndefined/Unknown
Pages (from-to)218-32
Number of pages15
JournalJournal of Controlled Release
Volume154
Issue number3
Publication statusPublished - 2011

Keywords

  • Farmacie/Biofarmaceutische wetenschappen (FARM)
  • Medical technology
  • Farmacie(FARM)
  • Biomedische technologie en medicijnen
  • Pharmacology

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