Host factors determine anti-GM1 response following oral challenge of chickens with Guillain-Barré syndrome derived Campylobacter jejuni strain GB11

C Wim Ang, Jeroen R Dijkstra, Marcel A de Klerk, Hubert Ph Endtz, Pieter A van Doorn, Bart C Jacobs, Suzan H M Jeurissen, Jaap A Wagenaar

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    BACKGROUND: Anti-ganglioside antibodies with a pathogenic potential are present in C. jejuni-associated Guillain-Barré syndrome (GBS) patients and are probably induced by molecular mimicry. Immunization studies in rabbits and mice have demonstrated that these anti-ganglioside antibodies can be induced using purified lipo-oligosaccharides (LOS) from C. jejuni in a strong adjuvant.

    METHODOLOGY/PRINCIPAL FINDINGS: To investigate whether natural colonization of chickens with a ganglioside-mimicking C. jejuni strain induces an anti-ganglioside response, and to investigate the diversity in anti-ganglioside response between and within genetically different chicken lines, we orally challenged chickens with different C. jejuni strains. Oral challenge of chickens with a C. jejuni strain from a GBS patient, containing a LOS that mimics ganglioside GM1, induced specific IgM and IgG anti-LOS and anti-GM1 antibodies. Inoculation of chickens with the Penner HS:3 serostrain, without a GM1-like structure, induced anti-LOS but no anti-ganglioside antibodies. We observed different patterns of anti-LOS/ganglioside response between and within the five strains of chickens.

    CONCLUSIONS: Natural infection of chickens with C. jejuni induces anti-ganglioside antibodies. The production of antibodies is governed by both microbial and host factors.

    Original languageEnglish
    Pages (from-to)e9820
    Number of pages1
    JournalPLoS One
    Volume5
    Issue number3
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Adjuvants, Immunologic
    • Animals
    • Campylobacter jejuni
    • Chickens
    • G(M1) Ganglioside
    • Guillain-Barre Syndrome
    • Immunization
    • Immunoglobulin G
    • Kinetics
    • Lipopolysaccharides
    • Mice
    • Molecular Mimicry
    • Rabbits
    • Species Specificity

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