Abstract
The analysis of intact protein assemblies in native-like states
by mass spectrometry offers a wealth of information on their
biochemical and biophysical properties. Here we show that
the Orbitrap mass analyzer can be used to measure protein
assemblies of molecular weights approaching one megadalton
with sensitivity down to the detection of single ions. Minor
instrumental modifications enabled the measurement of various
protein assemblies with outstanding mass-spectral resolution.
by mass spectrometry offers a wealth of information on their
biochemical and biophysical properties. Here we show that
the Orbitrap mass analyzer can be used to measure protein
assemblies of molecular weights approaching one megadalton
with sensitivity down to the detection of single ions. Minor
instrumental modifications enabled the measurement of various
protein assemblies with outstanding mass-spectral resolution.
| Original language | English |
|---|---|
| Pages (from-to) | 1084-1086 |
| Number of pages | 3 |
| Journal | Nature Methods |
| Volume | 9 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2013 |
Keywords
- Mass Spectrometry
- Structural Biology
- Biophysics
- Proteomics