Abstract
The two zinc fingers of the DNA binding domain of the human retinoic acid receptor-β were labelled with 113Cd. Two- and three-dimensional heteronuclear nuclear magnetic resonance (NMR) experiments show that the first eight conserved cysteine residues coordinate the two zinc ions tetrahedrally. The ninth conserved cysteine is not involved in metal coordination. In each finger one cysteine exhibits a heteronuclear 113Cd- 1H coupling constant substantially smaller than those of the other metal binding cysteines.
Original language | English |
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Pages (from-to) | 492-498 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 192 |
Issue number | 2 |
DOIs | |
Publication status | Published - 29 Jan 1993 |
Keywords
- cadmium
- retinoic acid binding protein
- zinc finger protein
- article
- binding site
- priority journal
- protein DNA binding
- proton nuclear magnetic resonance