Heparan sulfate facilitates Rift Valley fever virus entry into the cell

S.M. de Boer; J. Kortekaas; C.A.M. de Haan; P.J.M. Rottier; R.J.M. Moormann; B.J. Bosch

doi:10.1128/JVI.01364-12

Heparan sulfate facilitates Rift Valley fever virus entry into the cell

S.M. de Boer, J. Kortekaas, C.A.M. de Haan, P.J.M. Rottier, R.J.M. Moormann, B.J. Bosch

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Abstract

Rift Valley fever virus (RVFV), an emerging arthropod-borne pathogen, has a broad host and cell tropism. Here we report that the glycosaminoglycan heparan sulfate, abundantly present on the surface of most animal cells, is required for efficient entry of RVFV. Entry was significantly reduced by preincubating the virus inoculum with highly sulfated heparin, by enzymatic removal of heparan sulfate from cells and in cells genetically deficient in heparan sulfate synthesis.

Original language	English
Pages (from-to)	13767-13771
Number of pages	5
Journal	Journal of Virology
Volume	86
Issue number	24
DOIs	https://doi.org/10.1128/JVI.01364-12
Publication status	Published - 2012

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title = "Heparan sulfate facilitates Rift Valley fever virus entry into the cell",

abstract = "Rift Valley fever virus (RVFV), an emerging arthropod-borne pathogen, has a broad host and cell tropism. Here we report that the glycosaminoglycan heparan sulfate, abundantly present on the surface of most animal cells, is required for efficient entry of RVFV. Entry was significantly reduced by preincubating the virus inoculum with highly sulfated heparin, by enzymatic removal of heparan sulfate from cells and in cells genetically deficient in heparan sulfate synthesis.",

author = "{de Boer}, S.M. and J. Kortekaas and {de Haan}, C.A.M. and P.J.M. Rottier and R.J.M. Moormann and B.J. Bosch",

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AU - de Boer, S.M.

AU - Kortekaas, J.

AU - de Haan, C.A.M.

AU - Rottier, P.J.M.

AU - Moormann, R.J.M.

AU - Bosch, B.J.

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AB - Rift Valley fever virus (RVFV), an emerging arthropod-borne pathogen, has a broad host and cell tropism. Here we report that the glycosaminoglycan heparan sulfate, abundantly present on the surface of most animal cells, is required for efficient entry of RVFV. Entry was significantly reduced by preincubating the virus inoculum with highly sulfated heparin, by enzymatic removal of heparan sulfate from cells and in cells genetically deficient in heparan sulfate synthesis.

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DO - 10.1128/JVI.01364-12

M3 - Article

C2 - 23015725

SN - 0022-538X

VL - 86

SP - 13767

EP - 13771

JO - Journal of Virology

JF - Journal of Virology

IS - 24

ER -

Heparan sulfate facilitates Rift Valley fever virus entry into the cell

Abstract

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