Hemagglutinin receptor specificity and structural analyses of respiratory droplet-transmissible H5N1 viruses

Robert P de Vries, Xueyong Zhu, Ryan McBride, Alan Rigter, Anthony Hanson, Gongxun Zhong, Masato Hatta, Rui Xu, Wenli Yu, Yoshihiro Kawaoka, Cornelis A M de Haan, Ian A Wilson, James C Paulson

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Two ferret-adapted H5N1 viruses capable of respiratory droplet transmission have been reported with mutations in the hemagglutinin receptor-binding site and stalk domains. Glycan microarray analysis reveals that both viruses exhibit a strong shift toward binding to "human-type" α2-6 sialosides but with notable differences in fine specificity. Crystal structure analysis further shows that the stalk mutation causes no obvious perturbation of the receptor-binding pocket, consistent with its impact on hemagglutinin stability without affecting receptor specificity.

Original languageEnglish
Pages (from-to)768-73
Number of pages6
JournalJournal of Virology
Volume88
Issue number1
DOIs
Publication statusPublished - Jan 2014

Keywords

  • Hemagglutinins
  • Humans
  • Influenza A Virus, H5N1 Subtype

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