Heat shock protein expression in canine osteosarcoma

Mariarita Romanucci; Giuliana D'Amato; Daniela Malatesta; Laura Bongiovanni; Chiara Palmieri; Andrea Ciccarelli; Paolo Buracco; Emanuela Morello; Lorella Maniscalco; Raffaella De Maria; Marina Martano; Leonardo Della Salda

doi:10.1007/s12192-011-0294-2

Heat shock protein expression in canine osteosarcoma

Mariarita Romanucci, Giuliana D'Amato, Daniela Malatesta, Laura Bongiovanni, Chiara Palmieri, Andrea Ciccarelli, Paolo Buracco, Emanuela Morello, Lorella Maniscalco, Raffaella De Maria, Marina Martano, Leonardo Della Salda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Abnormal levels of heat shock proteins have been observed in a number of human neoplasms and demonstrate prognostic, predictive and therapeutic implications. Since osteosarcoma (OSA) in dogs provides an important model for the same disease in humans, the aim of this study was to evaluate the immunohistochemical expression of Hsp27, Hsp72, Hsp73 and Hsp90 in 18 samples of canine appendicular OSA, in relation to histological grade and overall survival (OS), in order to investigate their potential prognostic, predictive and/or therapeutic value. A semiquantitative method was used for the analysis of the results. Hsp27, Hsp73 and Hsp90 showed a variably intense, cytoplasmic and nuclear immunoreactivity that was not associated with histological type or grade. On the other hand, a high percentage of Hsp72 immunostaining was significantly associated with grade III (P<0.01) and a lack of immunolabelling was significantly correlated to a longer OS (P=0.006). Neoplastic emboli were occasionally positive for Hsp27, faintly immunoreactive for Hsp72 and intensely immunolabelled by Hsp73 and Hsp90. In conclusion, absence of Hsp72 immunosignal appears to be associated with a favourable prognosis whilst the widespread Hsp90 immunoreactivity detected in all tumour cases as well as in neoplastic emboli, suggests this protein could be targeted in the therapy of canine OSA, and likewise in its human counterpart.

Original language	English
Pages (from-to)	131-138
Number of pages	8
Journal	Cell Stress and Chaperones
Volume	17
Issue number	1
DOIs	https://doi.org/10.1007/s12192-011-0294-2
Publication status	Published - 1 Jan 2012

Funding

This work was presented at the VIII° meeting of the Italian Association of Veterinary Pathology (AIPVet), 15–17 June 2011, Padova, Italy. It has also been presented as a poster at the 29th meeting of the European Society of Veterinary Pathology (ESVP), 7–10 September 2011, Uppsala, Sweden. M.Romanucci.G.D’Amato.D.Malatesta.L.Bongiovanni. C. Palmieri.L. Della Salda (*) Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy e-mail: [email protected] Acknowledgements We thank Tania Bastow for the linguistic review of the manuscript. This work was completed with grant support from the Italian Ministry of University and Scientific Research, Rome, Italy.

Keywords

Animal model
Dog
Heat shock proteins
Osteosarcoma

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12192-011-0294-2

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title = "Heat shock protein expression in canine osteosarcoma",

abstract = "Abnormal levels of heat shock proteins have been observed in a number of human neoplasms and demonstrate prognostic, predictive and therapeutic implications. Since osteosarcoma (OSA) in dogs provides an important model for the same disease in humans, the aim of this study was to evaluate the immunohistochemical expression of Hsp27, Hsp72, Hsp73 and Hsp90 in 18 samples of canine appendicular OSA, in relation to histological grade and overall survival (OS), in order to investigate their potential prognostic, predictive and/or therapeutic value. A semiquantitative method was used for the analysis of the results. Hsp27, Hsp73 and Hsp90 showed a variably intense, cytoplasmic and nuclear immunoreactivity that was not associated with histological type or grade. On the other hand, a high percentage of Hsp72 immunostaining was significantly associated with grade III (P<0.01) and a lack of immunolabelling was significantly correlated to a longer OS (P=0.006). Neoplastic emboli were occasionally positive for Hsp27, faintly immunoreactive for Hsp72 and intensely immunolabelled by Hsp73 and Hsp90. In conclusion, absence of Hsp72 immunosignal appears to be associated with a favourable prognosis whilst the widespread Hsp90 immunoreactivity detected in all tumour cases as well as in neoplastic emboli, suggests this protein could be targeted in the therapy of canine OSA, and likewise in its human counterpart.",

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AU - Ciccarelli, Andrea

AU - Buracco, Paolo

AU - Morello, Emanuela

AU - Maniscalco, Lorella

AU - De Maria, Raffaella

AU - Martano, Marina

AU - Salda, Leonardo Della

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AB - Abnormal levels of heat shock proteins have been observed in a number of human neoplasms and demonstrate prognostic, predictive and therapeutic implications. Since osteosarcoma (OSA) in dogs provides an important model for the same disease in humans, the aim of this study was to evaluate the immunohistochemical expression of Hsp27, Hsp72, Hsp73 and Hsp90 in 18 samples of canine appendicular OSA, in relation to histological grade and overall survival (OS), in order to investigate their potential prognostic, predictive and/or therapeutic value. A semiquantitative method was used for the analysis of the results. Hsp27, Hsp73 and Hsp90 showed a variably intense, cytoplasmic and nuclear immunoreactivity that was not associated with histological type or grade. On the other hand, a high percentage of Hsp72 immunostaining was significantly associated with grade III (P<0.01) and a lack of immunolabelling was significantly correlated to a longer OS (P=0.006). Neoplastic emboli were occasionally positive for Hsp27, faintly immunoreactive for Hsp72 and intensely immunolabelled by Hsp73 and Hsp90. In conclusion, absence of Hsp72 immunosignal appears to be associated with a favourable prognosis whilst the widespread Hsp90 immunoreactivity detected in all tumour cases as well as in neoplastic emboli, suggests this protein could be targeted in the therapy of canine OSA, and likewise in its human counterpart.

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Heat shock protein expression in canine osteosarcoma

Abstract

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Keywords

UN SDGs

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