TY - JOUR
T1 - Gradient-purged isotope filter experiments for the detection of bound water in proteins
AU - Karimi-Nejad, Yasmin
AU - Löhr, Frank
AU - Schipper, Dick
AU - Rüterjans, Heinz
AU - Boelens, Rolf
PY - 1999/2/1
Y1 - 1999/2/1
N2 - A combination of gradient-purged isotope-filtered NMR experiments is
presented, which allows for the detection of long-lived bound water
molecules in proteins. The discrimination of direct water-protein
exchange from NOE effects between bound water and protein protons is
achieved by NOE/ROE cancellation during the mixing time in one of the
otherwise identical experiments. The method was applied successfully to
13C/ 15N-labelled serine protease PB92, and
allowed for the identification of 22 protein-water NOEs in this
269-residue enzyme.
AB - A combination of gradient-purged isotope-filtered NMR experiments is
presented, which allows for the detection of long-lived bound water
molecules in proteins. The discrimination of direct water-protein
exchange from NOE effects between bound water and protein protons is
achieved by NOE/ROE cancellation during the mixing time in one of the
otherwise identical experiments. The method was applied successfully to
13C/ 15N-labelled serine protease PB92, and
allowed for the identification of 22 protein-water NOEs in this
269-residue enzyme.
U2 - 10.1016/S0009-2614(98)01421-3
DO - 10.1016/S0009-2614(98)01421-3
M3 - Article
SN - 0009-2614
VL - 300
SP - 706
EP - 712
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 5
ER -