Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

C.J. Piek; E. Teske; M.W. van Leeuwen; M.J. Day

doi:10.1186/1751-0147-54-10

Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

C.J. Piek, E. Teske, M.W. van Leeuwen, M.J. Day

extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). Methods Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. Results The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. Conclusions The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT. Keywords: Haemolysis, Anaemia, Diagnostic test validation, Laboratory

Original language	English
Pages (from-to)	10.1186/1751-0147-54-10
Number of pages	8
Journal	Acta Veterinaria Scandinavica
Issue number	10
DOIs	https://doi.org/10.1186/1751-0147-54-10
Publication status	Published - 2012

Access to Document

10.1186/1751-0147-54-10

Good agreement of conventional and gel-based
Open Access (free)
Final published version, 378 KB

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296606/?tool=pubmed

Cite this

@article{c9c74c50fd3f42548ae930a0fb5890ae,

title = "Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia",

abstract = "Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). Methods Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. Results The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. Conclusions The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT. Keywords: Haemolysis, Anaemia, Diagnostic test validation, Laboratory",

author = "C.J. Piek and E. Teske and {van Leeuwen}, M.W. and M.J. Day",

year = "2012",

doi = "10.1186/1751-0147-54-10",

language = "English",

pages = "10.1186/1751--0147--54--10",

journal = "Acta Veterinaria Scandinavica",

issn = "0044-605X",

publisher = "BioMed Central",

number = "10",

}

TY - JOUR

T1 - Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

AU - Piek, C.J.

AU - Teske, E.

AU - van Leeuwen, M.W.

AU - Day, M.J.

PY - 2012

Y1 - 2012

N2 - Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). Methods Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. Results The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. Conclusions The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT. Keywords: Haemolysis, Anaemia, Diagnostic test validation, Laboratory

AB - Abstract Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). Methods Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. Results The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. Conclusions The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT. Keywords: Haemolysis, Anaemia, Diagnostic test validation, Laboratory

U2 - 10.1186/1751-0147-54-10

DO - 10.1186/1751-0147-54-10

M3 - Article

SN - 0044-605X

SP - 10.1186/1751-0147-54-10

JO - Acta Veterinaria Scandinavica

JF - Acta Veterinaria Scandinavica

IS - 10

ER -

Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia

Abstract

Access to Document

Fingerprint

Cite this