Genotyping by PCR, of Staphylococcus aureus strains, isolated from mammary glands of cows

Len J A Lipman, Arne De Nijs, Theo J G M Lam, Jules A. Rost, Linda Van Dijk, Ynte H. Schukken, Wim Gaastra*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    A total of 71 Staphylococcus aureus strains isolated from bovine mammary glands were identified and subtyped. The methods used to differentiate between the S. aureus isolates were the DNA polymorphism pattern after amplification with a Polymerase Chain Reaction using several primer combinations and phage typing. The DNA fingerprinting technique using RAPD, ERIC1R and ERIC primers proved to be useful in differentiating isolates of S. aureus. Differentiation of isolates using phage typing gave no additional information compared to the DNA technique. The outbreak of S. aureus in the herd studied was mainly caused by one S. aureus strain. Other strains were only fou;ld on three occasions, twice in subclinical infections and once from a case of clinical mastitis. In the Tatter case the dominant strain was isolated from a different quarter of the same cow. Four of the ten cows studied suffered from clinical mastitis. From those four cows, three remained infected with the same S. aureus strain despite antibiotic treatment.

    Original languageEnglish
    Pages (from-to)51-55
    Number of pages5
    JournalVeterinary Microbiology
    Volume48
    Issue number1-2
    DOIs
    Publication statusPublished - 1 Jan 1996

    Keywords

    • Dairy cattle
    • Epidemiology
    • Mastitis
    • Staphylococcus aureus

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