Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

Francesca Turroni; Francesca Bottacini; Elena Foroni; Imke Mulder; Jae-Han Kim; Aldert Zomer; Borja Sánchez; Alessandro Bidossi; Alberto Ferrarini; Vanessa Giubellini; Massimo Delledonne; Bernard Henrissat; Pedro Coutinho; Marco Oggioni; Gerald F Fitzgerald; David Mills; Abelardo Margolles; Denise Kelly; Douwe van Sinderen; Marco Ventura

doi:10.1073/pnas.1011100107

Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

Francesca Turroni, Francesca Bottacini, Elena Foroni, Imke Mulder, Jae-Han Kim, Aldert Zomer, Borja Sánchez, Alessandro Bidossi, Alberto Ferrarini, Vanessa Giubellini, Massimo Delledonne, Bernard Henrissat, Pedro Coutinho, Marco Oggioni, Gerald F Fitzgerald, David Mills, Abelardo Margolles, Denise Kelly, Douwe van Sinderen, Marco Ventura

University of Parma

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.

Original language	English
Pages (from-to)	19514-9
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	107
Issue number	45
DOIs	https://doi.org/10.1073/pnas.1011100107
Publication status	Published - 9 Nov 2010

Keywords

Bifidobacterium
Feces
Gene Expression Profiling
Genome, Bacterial
Genomics
Host-Pathogen Interactions
Humans
Infant, Newborn
Intestines
Metabolic Networks and Pathways
Molecular Sequence Data
Mucins
Multigene Family
Polysaccharides

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10.1073/pnas.1011100107

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Turroni, F., Bottacini, F., Foroni, E., Mulder, I., Kim, J.-H., Zomer, A., Sánchez, B., Bidossi, A., Ferrarini, A., Giubellini, V., Delledonne, M., Henrissat, B., Coutinho, P., Oggioni, M., Fitzgerald, G. F., Mills, D., Margolles, A., Kelly, D., van Sinderen, D., & Ventura, M. (2010). Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. Proceedings of the National Academy of Sciences of the United States of America, 107(45), 19514-9. https://doi.org/10.1073/pnas.1011100107

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title = "Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging",

abstract = "The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.",

keywords = "Bifidobacterium, Feces, Gene Expression Profiling, Genome, Bacterial, Genomics, Host-Pathogen Interactions, Humans, Infant, Newborn, Intestines, Metabolic Networks and Pathways, Molecular Sequence Data, Mucins, Multigene Family, Polysaccharides",

author = "Francesca Turroni and Francesca Bottacini and Elena Foroni and Imke Mulder and Jae-Han Kim and Aldert Zomer and Borja S{\'a}nchez and Alessandro Bidossi and Alberto Ferrarini and Vanessa Giubellini and Massimo Delledonne and Bernard Henrissat and Pedro Coutinho and Marco Oggioni and Fitzgerald, {Gerald F} and David Mills and Abelardo Margolles and Denise Kelly and {van Sinderen}, Douwe and Marco Ventura",

year = "2010",

month = nov,

day = "9",

doi = "10.1073/pnas.1011100107",

language = "English",

volume = "107",

pages = "19514--9",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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publisher = "National Academy of Sciences",

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Turroni, F, Bottacini, F, Foroni, E, Mulder, I, Kim, J-H, Zomer, A, Sánchez, B, Bidossi, A, Ferrarini, A, Giubellini, V, Delledonne, M, Henrissat, B, Coutinho, P, Oggioni, M, Fitzgerald, GF, Mills, D, Margolles, A, Kelly, D, van Sinderen, D & Ventura, M 2010, 'Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 45, pp. 19514-9. https://doi.org/10.1073/pnas.1011100107

Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. / Turroni, Francesca; Bottacini, Francesca; Foroni, Elena et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 45, 09.11.2010, p. 19514-9.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

AU - Turroni, Francesca

AU - Bottacini, Francesca

AU - Foroni, Elena

AU - Mulder, Imke

AU - Kim, Jae-Han

AU - Zomer, Aldert

AU - Sánchez, Borja

AU - Bidossi, Alessandro

AU - Ferrarini, Alberto

AU - Giubellini, Vanessa

AU - Delledonne, Massimo

AU - Henrissat, Bernard

AU - Coutinho, Pedro

AU - Oggioni, Marco

AU - Fitzgerald, Gerald F

AU - Mills, David

AU - Margolles, Abelardo

AU - Kelly, Denise

AU - van Sinderen, Douwe

AU - Ventura, Marco

PY - 2010/11/9

Y1 - 2010/11/9

N2 - The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.

AB - The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.

KW - Bifidobacterium

KW - Feces

KW - Gene Expression Profiling

KW - Genome, Bacterial

KW - Genomics

KW - Host-Pathogen Interactions

KW - Humans

KW - Infant, Newborn

KW - Intestines

KW - Metabolic Networks and Pathways

KW - Molecular Sequence Data

KW - Mucins

KW - Multigene Family

KW - Polysaccharides

U2 - 10.1073/pnas.1011100107

DO - 10.1073/pnas.1011100107

M3 - Article

C2 - 20974960

SN - 0027-8424

VL - 107

SP - 19514

EP - 19519

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 45

ER -

Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

Abstract

Keywords

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