Genes for elongation factor EF-1α in the brine shrimp Artemia

Formijn J. van Hemert; Johannes A. Lenstra; Wim Möller

doi:10.1016/0014-5793(83)80564-X

Genes for elongation factor EF-1α in the brine shrimp Artemia

Formijn J. van Hemert^*
, Johannes A. Lenstra
, Wim Möller

^*Corresponding author for this work

Sylvius Laboratoria

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A plasmid carrying a cDNA sequence coding for elongation factor EF-1α from Artemia was used to probe blots of mRNA and chromosomal DNA from Artemia. A messenger length for EF-1α corresponding to 1850 nucleotides was found. Southern blots pointed to a limited number (1-4) genes, coding for EF-1α. From an Artemia gene library a recombinant phage was isolated, which contains genomic sequences of EF-1α. S1-nuclease mapping indicated the presence of intervening sequences within this cloned gene.

Original language	English
Pages (from-to)	295-299
Number of pages	5
Journal	FEBS Letters
Volume	157
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(83)80564-X
Publication status	Published - 4 Jul 1983

Keywords

Artemia
Elongation factor EF-1α
Gene library
Northern blot
Southern blot

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10.1016/0014-5793(83)80564-X

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title = "Genes for elongation factor EF-1α in the brine shrimp Artemia",

abstract = "A plasmid carrying a cDNA sequence coding for elongation factor EF-1α from Artemia was used to probe blots of mRNA and chromosomal DNA from Artemia. A messenger length for EF-1α corresponding to 1850 nucleotides was found. Southern blots pointed to a limited number (1-4) genes, coding for EF-1α. From an Artemia gene library a recombinant phage was isolated, which contains genomic sequences of EF-1α. S1-nuclease mapping indicated the presence of intervening sequences within this cloned gene.",

keywords = "Artemia, Elongation factor EF-1α, Gene library, Northern blot, Southern blot",

author = "\{van Hemert\}, \{Formijn J.\} and Lenstra, \{Johannes A.\} and Wim M{\"o}ller",

year = "1983",

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doi = "10.1016/0014-5793(83)80564-X",

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AU - van Hemert, Formijn J.

AU - Lenstra, Johannes A.

AU - Möller, Wim

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Y1 - 1983/7/4

N2 - A plasmid carrying a cDNA sequence coding for elongation factor EF-1α from Artemia was used to probe blots of mRNA and chromosomal DNA from Artemia. A messenger length for EF-1α corresponding to 1850 nucleotides was found. Southern blots pointed to a limited number (1-4) genes, coding for EF-1α. From an Artemia gene library a recombinant phage was isolated, which contains genomic sequences of EF-1α. S1-nuclease mapping indicated the presence of intervening sequences within this cloned gene.

AB - A plasmid carrying a cDNA sequence coding for elongation factor EF-1α from Artemia was used to probe blots of mRNA and chromosomal DNA from Artemia. A messenger length for EF-1α corresponding to 1850 nucleotides was found. Southern blots pointed to a limited number (1-4) genes, coding for EF-1α. From an Artemia gene library a recombinant phage was isolated, which contains genomic sequences of EF-1α. S1-nuclease mapping indicated the presence of intervening sequences within this cloned gene.

KW - Artemia

KW - Elongation factor EF-1α

KW - Gene library

KW - Northern blot

KW - Southern blot

UR - https://www.scopus.com/pages/publications/0021094810

U2 - 10.1016/0014-5793(83)80564-X

DO - 10.1016/0014-5793(83)80564-X

M3 - Article

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SP - 295

EP - 299

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Genes for elongation factor EF-1α in the brine shrimp Artemia

Abstract

Keywords

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