Generic detection of coronaviruses and differentiation at the prototype strain level by RT-PCR and non-fluorescent low density microarray

L.K. de Souza Luna; V. Heiser; N. Regamey; M. Panning; J.F. Drexler; S. Mulangu; L. Poon; S. Baumgarte; B.J. Haijema; L. Kaiser; C. Drosten

doi:10.1128/JCM.02426-06

Generic detection of coronaviruses and differentiation at the prototype strain level by RT-PCR and non-fluorescent low density microarray

L.K. de Souza Luna, V. Heiser, N. Regamey, M. Panning, J.F. Drexler, S. Mulangu, L. Poon, S. Baumgarte, B.J. Haijema, L. Kaiser, C. Drosten

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

Original language	Undefined/Unknown
Pages (from-to)	1049-1052
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1128/JCM.02426-06
Publication status	Published - 2007

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10.1128/JCM.02426-06

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de Souza Luna, L. K., Heiser, V., Regamey, N., Panning, M., Drexler, J. F., Mulangu, S., Poon, L., Baumgarte, S., Haijema, B. J., Kaiser, L., & Drosten, C. (2007). Generic detection of coronaviruses and differentiation at the prototype strain level by RT-PCR and non-fluorescent low density microarray. Journal of Clinical Microbiology, 45(3), 1049-1052. https://doi.org/10.1128/JCM.02426-06

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de Souza Luna, LK, Heiser, V, Regamey, N, Panning, M, Drexler, JF, Mulangu, S, Poon, L, Baumgarte, S, Haijema, BJ, Kaiser, L & Drosten, C 2007, 'Generic detection of coronaviruses and differentiation at the prototype strain level by RT-PCR and non-fluorescent low density microarray', Journal of Clinical Microbiology, vol. 45, no. 3, pp. 1049-1052. https://doi.org/10.1128/JCM.02426-06

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AU - de Souza Luna, L.K.

AU - Heiser, V.

AU - Regamey, N.

AU - Panning, M.

AU - Drexler, J.F.

AU - Mulangu, S.

AU - Poon, L.

AU - Baumgarte, S.

AU - Haijema, B.J.

AU - Kaiser, L.

AU - Drosten, C.

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