Generation of mixed murine organoids to model cellular interactions.

Ana Krotenberg Garcia; Jacco van Rheenen; Saskia Suijkerbuijk

doi:10.1016/j.xpro.2021.100997

Generation of mixed murine organoids to model cellular interactions.

Ana Krotenberg Garcia, Jacco van Rheenen, Saskia Suijkerbuijk

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

Original language	English
Article number	100997
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100997
Publication status	Published - 8 Dec 2021
Externally published	Yes

Keywords

Cancer
Cell Biology
Cell culture
Microscopy
Organoids

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10.1016/j.xpro.2021.100997Licence: CC BY

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title = "Generation of mixed murine organoids to model cellular interactions.",

abstract = "Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).",

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author = "{Krotenberg Garcia}, Ana and {van Rheenen}, Jacco and Saskia Suijkerbuijk",

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AU - van Rheenen, Jacco

AU - Suijkerbuijk, Saskia

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N2 - Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

AB - Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

KW - Cancer

KW - Cell Biology

KW - Cell culture

KW - Microscopy

KW - Organoids

UR - https://europepmc.org/articles/PMC8666359

U2 - 10.1016/j.xpro.2021.100997

DO - 10.1016/j.xpro.2021.100997

M3 - Article

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JO - STAR Protocols

JF - STAR Protocols

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ER -

Generation of mixed murine organoids to model cellular interactions.

Abstract

Keywords

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