Gene Expression Profiling of Cecropin B-Resistant Haemophilus parasuis

Chunmei Wang, Fangzhou Chen, Han Hu, Wentao Li, Yang Wang, Pin Chen, Yingyu Liu, Xugang Ku, Qigai He*, Huanchun Chen, Feiqun Xue

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Synthetically designed antimicrobial peptides (AMPs) present the potential of replacing antibiotics in the treatment of bacterial infections. However, microbial resistance to AMPs has been reported and little is known regarding the underlying mechanism of such resistance. The naturally occurring AMP cecropin B (CB) disrupts the anionic cell membranes of Gram-negative bacteria. In this study, CB resistance (CBR) was induced in Haemophilus parasuis SH0165 by exposing it to a series of CB concentrations. The CB-resistant H. parasuis strains CBR30 and CBR30-50 were obtained. The growth curves of SH0165 and CBR30 showed that CBR30 displayed lower growth rates than SH0165. The result of transmission electron microscopy showed cell membranes of the CB-resistant CBR30 and CBR30-50 were smoother than SH0165. Microarrays detected 257 upregulated and 254 downregulated genes covering 20 clusters of orthologous groups (COGs) of the CB-resistant CBR30 compared with SH0165 (>1.5-fold change, p <0.05). Sixty genes were affected in CBR30-50 covering 18 COGs, with 28 upregulated and 32 downregulated genes. Under the COG function classification, the majority of affected genes in the CB-resistant CBR30 and CBR30-50 belong to the category of inorganic ion transport, amino acid transport, and metabolism. The microarray results were validated by real-time quantitative reverse transcription PCR. This study may provide useful guidance for understanding the molecular mechanism underlying H. parasuis resistance to CB. (C) 2014 S. Karger AG, Basel

    Original languageEnglish
    Pages (from-to)120-129
    Number of pages10
    JournalJournal of Molecular Microbiology and Biotechnology
    Volume24
    Issue number2
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Antimicrobial peptides
    • Cecropin B
    • DNA microarray
    • Haemophilus parasuis
    • Transcriptional profiling
    • ANTIMICROBIAL PEPTIDES
    • BACTERIAL PATHOGENS
    • ESCHERICHIA-COLI
    • ANTIBIOTICS
    • MECHANISM

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