TY - JOUR
T1 - Gene expression of transformed lens cells
AU - Lenstra, Johannes A.
AU - Hukkelhoven, Math W A C
AU - Gròeneveld, Ap A.
AU - Smits, Rob A M M
AU - Weterings, Peter J J M
AU - Bloemendal, Hans
PY - 1982/1/1
Y1 - 1982/1/1
N2 - In addition to our SV40-transformed hamster cell line (Bloemendal et al. 1980), two otherlenticular cell lines have been established. One was obtained by transformation of hamster lens cells with the carcinogen 3-methylcholanthrene, the other one by transformation of calf lens cells with SV40. The transformed cells revealed alterations in growth pattern and morphology, but only minor changes in gene expression. However, crystallin synthesis ceased in all cases. We hypothesize that elongation of the lens cells in vitro reflects a transition into fibroblast-like cells.
AB - In addition to our SV40-transformed hamster cell line (Bloemendal et al. 1980), two otherlenticular cell lines have been established. One was obtained by transformation of hamster lens cells with the carcinogen 3-methylcholanthrene, the other one by transformation of calf lens cells with SV40. The transformed cells revealed alterations in growth pattern and morphology, but only minor changes in gene expression. However, crystallin synthesis ceased in all cases. We hypothesize that elongation of the lens cells in vitro reflects a transition into fibroblast-like cells.
KW - lens cell culture
KW - SV40
KW - transformation
KW - tumor cells
KW - two-dimensional gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0020421967&partnerID=8YFLogxK
U2 - 10.1016/S0014-4835(82)80069-9
DO - 10.1016/S0014-4835(82)80069-9
M3 - Article
C2 - 7151889
AN - SCOPUS:0020421967
SN - 0014-4835
VL - 35
SP - 549
EP - 554
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 6
ER -