Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs

Wouter Schuurman; Peter A Levett; Michiel W Pot; Paul René van Weeren; Wouter J A Dhert; Dietmar W Hutmacher; Ferry P W Melchels; Travis J Klein; Jos Malda

doi:10.1002/mabi.201200471

Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs

Wouter Schuurman, Peter A Levett, Michiel W Pot, Paul René van Weeren, Wouter J A Dhert, Dietmar W Hutmacher, Ferry P W Melchels, Travis J Klein, Jos Malda

Faculty of Veterinary Medicine

University Medical Center Utrecht

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 °C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

Original language	English
Pages (from-to)	551-561
Number of pages	11
Journal	Macromolecular Bioscience
Volume	13
Issue number	5
DOIs	https://doi.org/10.1002/mabi.201200471
Publication status	Published - May 2013

Bibliographical note

Keywords

Acrylamides
Animals
Biocompatible Materials
Bioprinting
Cartilage
Cell Survival
Compressive Strength
Cross-Linking Reagents
DNA
Gelatin
Glycosaminoglycans
Horses
Hyaluronic Acid
Hydrogels
Immunohistochemistry
Materials Testing
Mechanical Phenomena
Temperature
Tissue Engineering
Tissue Scaffolds
Ultraviolet Rays

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10.1002/mabi.201200471

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265SchuurmanMacromolBioSc(Gelma)2013
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Embargo ends: 1/01/50

Cite this

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title = "Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs",

abstract = "Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 °C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.",

keywords = "Acrylamides, Animals, Biocompatible Materials, Bioprinting, Cartilage, Cell Survival, Compressive Strength, Cross-Linking Reagents, DNA, Gelatin, Glycosaminoglycans, Horses, Hyaluronic Acid, Hydrogels, Immunohistochemistry, Materials Testing, Mechanical Phenomena, Temperature, Tissue Engineering, Tissue Scaffolds, Ultraviolet Rays",

author = "Wouter Schuurman and Levett, \{Peter A\} and Pot, \{Michiel W\} and \{van Weeren\}, \{Paul Ren{\'e}\} and Dhert, \{Wouter J A\} and Hutmacher, \{Dietmar W\} and Melchels, \{Ferry P W\} and Klein, \{Travis J\} and Jos Malda",

note = "Copyright {\textcopyright} 2013 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

year = "2013",

month = may,

doi = "10.1002/mabi.201200471",

language = "English",

volume = "13",

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journal = "Macromolecular Bioscience",

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publisher = "Wiley",

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AU - Schuurman, Wouter

AU - Levett, Peter A

AU - Pot, Michiel W

AU - van Weeren, Paul René

AU - Dhert, Wouter J A

AU - Hutmacher, Dietmar W

AU - Melchels, Ferry P W

AU - Klein, Travis J

AU - Malda, Jos

PY - 2013/5

Y1 - 2013/5

N2 - Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 °C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

AB - Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 °C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

KW - Acrylamides

KW - Animals

KW - Biocompatible Materials

KW - Bioprinting

KW - Cartilage

KW - Cell Survival

KW - Compressive Strength

KW - Cross-Linking Reagents

KW - DNA

KW - Gelatin

KW - Glycosaminoglycans

KW - Horses

KW - Hyaluronic Acid

KW - Hydrogels

KW - Immunohistochemistry

KW - Materials Testing

KW - Mechanical Phenomena

KW - Temperature

KW - Tissue Engineering

KW - Tissue Scaffolds

KW - Ultraviolet Rays

U2 - 10.1002/mabi.201200471

DO - 10.1002/mabi.201200471

M3 - Article

C2 - 23420700

SN - 1616-5187

VL - 13

SP - 551

EP - 561

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 5

ER -

Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs

Abstract

Bibliographical note

Keywords

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Cite this