Future Directions of the Prokaryotic Chromosome Field

E. A. Abbondanzieri; A. B. Badrinarayanan; D. Barillà; S. D. Bell; F. Blombach; J. Y. Bouet; S. Bulgheresi; Q. A.D. Cao; R. T. Dame; C. Dekker; M. Demuysere; O. Espéli; P. C.M. Fogg; P. L. Freddolino; M. Ganji; T. M. Gerson; D. C. Grainger; L. W. Hamoen; J. Harju; A. Hocher; C. M. Hustmyer; J. K. Kaljevic; M. K. Karney; N. Kleckner; G. Laloux; R. Landick; V. S. Lioy; W. L. Liu; C. L. Liu; J. Mäkelä; A. S. Meyer; A. Noy; M. P. Pineau; K. Premrajka; L. R. Racki; F. Z.M. Rashid; K. Schnetz; S. Schwab; M. Tišma; A. I. van der Sijs; T. van Heesch; R. van Raaphorst; J. Vreede; A. W. Walker; J. C. Walter; S. C. Weber; P. A. Wiggins; H. J. Wing; J. Xiao; Z. Zhang

doi:10.1111/mmi.15347

Future Directions of the Prokaryotic Chromosome Field

E. A. Abbondanzieri, A. B. Badrinarayanan, D. Barillà^*, S. D. Bell, F. Blombach, J. Y. Bouet, S. Bulgheresi, Q. A.D. Cao, R. T. Dame^*, C. Dekker, M. Demuysere, O. Espéli^*, P. C.M. Fogg, P. L. Freddolino, M. Ganji, T. M. Gerson, D. C. Grainger^*, L. W. Hamoen, J. Harju, A. HocherC. M. Hustmyer, J. K. Kaljevic, M. K. Karney, N. Kleckner, G. Laloux, R. Landick, V. S. Lioy, W. L. Liu, C. L. Liu, J. Mäkelä, A. S. Meyer, A. Noy, M. P. Pineau, K. Premrajka, L. R. Racki, F. Z.M. Rashid, K. Schnetz, S. Schwab, M. Tišma, A. I. van der Sijs, T. van Heesch, R. van Raaphorst, J. Vreede, A. W. Walker, J. C. Walter, S. C. Weber, P. A. Wiggins^*, H. J. Wing, J. Xiao, Z. Zhang

^*Corresponding author for this work

Sub Physical and Colloid Chemistry

Research output: Contribution to journal › Editorial › Academic › peer-review

Abstract

In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around current challenges for the field, how these might be addressed, and how the field is likely to develop over the next 10 years. The Lorentz Center staff facilitated these discussions via tools aimed at optimizing productive interactions. This Perspective article is a summary of these discussions and reflects the state-of-the-art of the field. It is expected to be of help to colleagues in advancing their own research related to prokaryotic chromosomes and inspiring novel interdisciplinary collaborations. This forward-looking perspective highlights the open questions driving current research and builds on the impressive recent progress in these areas as represented by the accompanying reviews, perspectives, and research articles in this issue. These articles underline the multi-disciplinary nature of the field, the multiple length scales at which chromatin is studied in vitro and in and highlight the differences and similarities of bacterial and archaeal chromatin and chromatin-associated processes.

Original language	English
Pages (from-to)	89-100
Number of pages	12
Journal	Molecular Microbiology
Volume	123
Issue number	2
DOIs	https://doi.org/10.1111/mmi.15347
Publication status	Published - Feb 2025

Bibliographical note

Publisher Copyright:
© 2025 John Wiley & Sons Ltd.

Funding

The authors would like to thank the Lorentz Center (Leiden, The Netherlands) for hosting the workshop "Biology and physics of the prokaryotic chromosome" from September 11-15, 2023. Specifically, the "Open Space Technology" sessions facilitated by Lorentz Center staff were instrumental in inspiring and shaping the discussions summarized in this article. The Lorentz Center is funded by NWO and Leiden University.

Funders	Funder number
The Lorentz Center is funded by the Dutch Research Council (NWO) and Leiden University	2023
NWO and Leiden University

Keywords

archaeal chromatin
archaeal chromosome
bacterial chromatin
bacterial chromosome
nucleoid

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Abbondanzieri, E. A., Badrinarayanan, A. B., Barillà, D., Bell, S. D., Blombach, F., Bouet, J. Y., Bulgheresi, S., Cao, Q. A. D., Dame, R. T., Dekker, C., Demuysere, M., Espéli, O., Fogg, P. C. M., Freddolino, P. L., Ganji, M., Gerson, T. M., Grainger, D. C., Hamoen, L. W., Harju, J., ... Zhang, Z. (2025). Future Directions of the Prokaryotic Chromosome Field. Molecular Microbiology, 123(2), 89-100. https://doi.org/10.1111/mmi.15347

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title = "Future Directions of the Prokaryotic Chromosome Field",

abstract = "In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around current challenges for the field, how these might be addressed, and how the field is likely to develop over the next 10 years. The Lorentz Center staff facilitated these discussions via tools aimed at optimizing productive interactions. This Perspective article is a summary of these discussions and reflects the state-of-the-art of the field. It is expected to be of help to colleagues in advancing their own research related to prokaryotic chromosomes and inspiring novel interdisciplinary collaborations. This forward-looking perspective highlights the open questions driving current research and builds on the impressive recent progress in these areas as represented by the accompanying reviews, perspectives, and research articles in this issue. These articles underline the multi-disciplinary nature of the field, the multiple length scales at which chromatin is studied in vitro and in and highlight the differences and similarities of bacterial and archaeal chromatin and chromatin-associated processes.",

keywords = "archaeal chromatin, archaeal chromosome, bacterial chromatin, bacterial chromosome, nucleoid",

author = "Abbondanzieri, {E. A.} and Badrinarayanan, {A. B.} and D. Barill{\`a} and Bell, {S. D.} and F. Blombach and Bouet, {J. Y.} and S. Bulgheresi and Cao, {Q. A.D.} and Dame, {R. T.} and C. Dekker and M. Demuysere and O. Esp{\'e}li and Fogg, {P. C.M.} and Freddolino, {P. L.} and M. Ganji and Gerson, {T. M.} and Grainger, {D. C.} and Hamoen, {L. W.} and J. Harju and A. Hocher and Hustmyer, {C. M.} and Kaljevic, {J. K.} and Karney, {M. K.} and N. Kleckner and G. Laloux and R. Landick and Lioy, {V. S.} and Liu, {W. L.} and Liu, {C. L.} and J. M{\"a}kel{\"a} and Meyer, {A. S.} and A. Noy and Pineau, {M. P.} and K. Premrajka and Racki, {L. R.} and Rashid, {F. Z.M.} and K. Schnetz and S. Schwab and M. Ti{\v s}ma and {van der Sijs}, {A. I.} and {van Heesch}, T. and {van Raaphorst}, R. and J. Vreede and Walker, {A. W.} and Walter, {J. C.} and Weber, {S. C.} and Wiggins, {P. A.} and Wing, {H. J.} and J. Xiao and Z. Zhang",

note = "Publisher Copyright: {\textcopyright} 2025 John Wiley & Sons Ltd.",

year = "2025",

month = feb,

doi = "10.1111/mmi.15347",

language = "English",

volume = "123",

pages = "89--100",

journal = "Molecular Microbiology",

issn = "0950-382X",

publisher = "John Wiley and Sons Inc.",

number = "2",

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Abbondanzieri, EA, Badrinarayanan, AB, Barillà, D, Bell, SD, Blombach, F, Bouet, JY, Bulgheresi, S, Cao, QAD, Dame, RT, Dekker, C, Demuysere, M, Espéli, O, Fogg, PCM, Freddolino, PL, Ganji, M, Gerson, TM, Grainger, DC, Hamoen, LW, Harju, J, Hocher, A, Hustmyer, CM, Kaljevic, JK, Karney, MK, Kleckner, N, Laloux, G, Landick, R, Lioy, VS, Liu, WL, Liu, CL, Mäkelä, J, Meyer, AS, Noy, A, Pineau, MP, Premrajka, K, Racki, LR, Rashid, FZM, Schnetz, K, Schwab, S, Tišma, M, van der Sijs, AI, van Heesch, T, van Raaphorst, R, Vreede, J, Walker, AW, Walter, JC, Weber, SC, Wiggins, PA, Wing, HJ, Xiao, J & Zhang, Z 2025, 'Future Directions of the Prokaryotic Chromosome Field', Molecular Microbiology, vol. 123, no. 2, pp. 89-100. https://doi.org/10.1111/mmi.15347

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T1 - Future Directions of the Prokaryotic Chromosome Field

AU - Abbondanzieri, E. A.

AU - Badrinarayanan, A. B.

AU - Barillà, D.

AU - Bell, S. D.

AU - Blombach, F.

AU - Bouet, J. Y.

AU - Bulgheresi, S.

AU - Cao, Q. A.D.

AU - Dame, R. T.

AU - Dekker, C.

AU - Demuysere, M.

AU - Espéli, O.

AU - Fogg, P. C.M.

AU - Freddolino, P. L.

AU - Ganji, M.

AU - Gerson, T. M.

AU - Grainger, D. C.

AU - Hamoen, L. W.

AU - Harju, J.

AU - Hocher, A.

AU - Hustmyer, C. M.

AU - Kaljevic, J. K.

AU - Karney, M. K.

AU - Kleckner, N.

AU - Laloux, G.

AU - Landick, R.

AU - Lioy, V. S.

AU - Liu, W. L.

AU - Liu, C. L.

AU - Mäkelä, J.

AU - Meyer, A. S.

AU - Noy, A.

AU - Pineau, M. P.

AU - Premrajka, K.

AU - Racki, L. R.

AU - Rashid, F. Z.M.

AU - Schnetz, K.

AU - Schwab, S.

AU - Tišma, M.

AU - van der Sijs, A. I.

AU - van Heesch, T.

AU - van Raaphorst, R.

AU - Vreede, J.

AU - Walker, A. W.

AU - Walter, J. C.

AU - Weber, S. C.

AU - Wiggins, P. A.

AU - Wing, H. J.

AU - Xiao, J.

AU - Zhang, Z.

PY - 2025/2

Y1 - 2025/2

N2 - In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around current challenges for the field, how these might be addressed, and how the field is likely to develop over the next 10 years. The Lorentz Center staff facilitated these discussions via tools aimed at optimizing productive interactions. This Perspective article is a summary of these discussions and reflects the state-of-the-art of the field. It is expected to be of help to colleagues in advancing their own research related to prokaryotic chromosomes and inspiring novel interdisciplinary collaborations. This forward-looking perspective highlights the open questions driving current research and builds on the impressive recent progress in these areas as represented by the accompanying reviews, perspectives, and research articles in this issue. These articles underline the multi-disciplinary nature of the field, the multiple length scales at which chromatin is studied in vitro and in and highlight the differences and similarities of bacterial and archaeal chromatin and chromatin-associated processes.

AB - In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around current challenges for the field, how these might be addressed, and how the field is likely to develop over the next 10 years. The Lorentz Center staff facilitated these discussions via tools aimed at optimizing productive interactions. This Perspective article is a summary of these discussions and reflects the state-of-the-art of the field. It is expected to be of help to colleagues in advancing their own research related to prokaryotic chromosomes and inspiring novel interdisciplinary collaborations. This forward-looking perspective highlights the open questions driving current research and builds on the impressive recent progress in these areas as represented by the accompanying reviews, perspectives, and research articles in this issue. These articles underline the multi-disciplinary nature of the field, the multiple length scales at which chromatin is studied in vitro and in and highlight the differences and similarities of bacterial and archaeal chromatin and chromatin-associated processes.

KW - archaeal chromatin

KW - archaeal chromosome

KW - bacterial chromatin

KW - bacterial chromosome

KW - nucleoid

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U2 - 10.1111/mmi.15347

DO - 10.1111/mmi.15347

M3 - Editorial

AN - SCOPUS:85218347161

SN - 0950-382X

VL - 123

SP - 89

EP - 100

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 2

ER -

Future Directions of the Prokaryotic Chromosome Field

Abstract

Bibliographical note

Funding

Keywords

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