Fungal glycoside hydrolase family 44 xyloglucanases are restricted to the phylum Basidiomycota and show a distinct xyloglucan cleavage pattern

Peicheng Sun; Xinxin Li; Adiphol Dilokpimol; Bernard Henrissat; Ronald P de Vries; Mirjam A Kabel; Miia R Mäkelä

doi:10.1016/j.isci.2021.103666

Fungal glycoside hydrolase family 44 xyloglucanases are restricted to the phylum Basidiomycota and show a distinct xyloglucan cleavage pattern

Peicheng Sun, Xinxin Li, Adiphol Dilokpimol, Bernard Henrissat, Ronald P de Vries, Mirjam A Kabel, Miia R Mäkelä

Molecular Plant Physiology

Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, PO Box 80163, 3508 TD Utrecht, the Netherlands; Department of Animal Sciences, Animal Nutrition Group, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands.
Utrecht University, University Medical Center Utrecht
Technical University of Denmark
Helsinki University of the Arts

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Xyloglucan is a prominent matrix heteropolysaccharide binding to cellulose microfibrils in primary plant cell walls. Hence, the hydrolysis of xyloglucan facilitates the overall lignocellulosic biomass degradation. Xyloglucanases (XEGs) are key enzymes classified in several glycoside hydrolase (GH) families. So far, family GH44 has been shown to contain bacterial XEGs only. Detailed genome analysis revealed GH44 members in fungal species from the phylum Basidiomycota, but not in other fungi, which we hypothesized to also be XEGs. Two GH44 enzymes from Dichomitus squalens and Pleurotus ostreatus were heterologously produced and characterized. They exhibited XEG activity and displayed a hydrolytic cleavage pattern different from that observed in fungal XEGs from other GH families. Specifically, the fungal GH44 XEGs were not hindered by substitution of neighboring glucosyl units and generated various "XXXG-type," "GXXX(G)-type," and "XXX-type" oligosaccharides. Overall, these fungal GH44 XEGs represent a novel class of enzymes for plant biomass conversion and valorization.

Original language	English
Article number	103666
Pages (from-to)	1-14
Journal	iScience
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/j.isci.2021.103666
Publication status	Published - 21 Jan 2022

Bibliographical note

Funding Information:
Alisa Shubina, BSc, is acknowledged for her skillful technical assistance. This work was supported by the China Scholarship Council (grant no. 201803250066 to X.L.) and the Academy of Finland (grant no. 308284 to M.R.M.).

Publisher Copyright:
© 2021 The Authors

Keywords

Biomass
Enzymology
Mycology

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PIIS2589004221016369Final published version, 2.78 MBLicence: CC BY-NC-ND

Cite this

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abstract = "Xyloglucan is a prominent matrix heteropolysaccharide binding to cellulose microfibrils in primary plant cell walls. Hence, the hydrolysis of xyloglucan facilitates the overall lignocellulosic biomass degradation. Xyloglucanases (XEGs) are key enzymes classified in several glycoside hydrolase (GH) families. So far, family GH44 has been shown to contain bacterial XEGs only. Detailed genome analysis revealed GH44 members in fungal species from the phylum Basidiomycota, but not in other fungi, which we hypothesized to also be XEGs. Two GH44 enzymes from Dichomitus squalens and Pleurotus ostreatus were heterologously produced and characterized. They exhibited XEG activity and displayed a hydrolytic cleavage pattern different from that observed in fungal XEGs from other GH families. Specifically, the fungal GH44 XEGs were not hindered by substitution of neighboring glucosyl units and generated various {"}XXXG-type,{"} {"}GXXX(G)-type,{"} and {"}XXX-type{"} oligosaccharides. Overall, these fungal GH44 XEGs represent a novel class of enzymes for plant biomass conversion and valorization.",

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note = "Funding Information: Alisa Shubina, BSc, is acknowledged for her skillful technical assistance. This work was supported by the China Scholarship Council (grant no. 201803250066 to X.L.) and the Academy of Finland (grant no. 308284 to M.R.M.). Publisher Copyright: {\textcopyright} 2021 The Authors",

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AU - Henrissat, Bernard

AU - de Vries, Ronald P

AU - Kabel, Mirjam A

AU - Mäkelä, Miia R

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PY - 2022/1/21

Y1 - 2022/1/21

N2 - Xyloglucan is a prominent matrix heteropolysaccharide binding to cellulose microfibrils in primary plant cell walls. Hence, the hydrolysis of xyloglucan facilitates the overall lignocellulosic biomass degradation. Xyloglucanases (XEGs) are key enzymes classified in several glycoside hydrolase (GH) families. So far, family GH44 has been shown to contain bacterial XEGs only. Detailed genome analysis revealed GH44 members in fungal species from the phylum Basidiomycota, but not in other fungi, which we hypothesized to also be XEGs. Two GH44 enzymes from Dichomitus squalens and Pleurotus ostreatus were heterologously produced and characterized. They exhibited XEG activity and displayed a hydrolytic cleavage pattern different from that observed in fungal XEGs from other GH families. Specifically, the fungal GH44 XEGs were not hindered by substitution of neighboring glucosyl units and generated various "XXXG-type," "GXXX(G)-type," and "XXX-type" oligosaccharides. Overall, these fungal GH44 XEGs represent a novel class of enzymes for plant biomass conversion and valorization.

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KW - Mycology

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Fungal glycoside hydrolase family 44 xyloglucanases are restricted to the phylum Basidiomycota and show a distinct xyloglucan cleavage pattern

Abstract

Bibliographical note

Keywords

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