Frozen-thawed ampullary cell monolayer improves bovine embryo in vitro development and quality

Anise Asaadi, Mojtaba Kafi*, Hadi Atashi, Mehdi Azari, Miel Hostens

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    The aim of this study was to evaluate the effects of different timing for frozen-thawed bovine ampullary epithelial cell (BAEC) and bovine oviductal epithelial cell (BOEC) co-culture on the development and quality of bovine embryos produced in vitro. Embryo development was assessed by day 8 blastocyst yield, whereas embryo quality was determined using blastocyst differential cell count, cryotolerance and the expression of selected genes related to embryo quality. The results showed that the presence of BAECs during the last 6 h of in vitro maturation (IVM) increased blastocyst yield and survival of the vitrified-warmed blastocysts. In addition, embryos produced in the presence of BAECs during the last 6 h of IVM or in the presence of BOECs during the first 4 days of in vitro culture (IVC) showed a greater number of trophectoderm cells and a greater inner cell mass. In terms of gene expression, IFN-T was downregulated and PLAC8, AQP3 and ATP1A1 were upregulated in the presence of the BAECs during the last 6 h of the IVM and/or in the presence of BOECs during the first 4 days of IVC. In conclusion, co-culturing bovine oocytes with a frozen-thawed ampullary cell monolayer during the last 6 h of maturation increased blastocyst yield and quality.

    Original languageEnglish
    Pages (from-to)337-346
    Number of pages10
    JournalZygote
    Volume27
    Issue number5
    Early online date13 Aug 2019
    DOIs
    Publication statusPublished - 1 Oct 2019

    Keywords

    • Ampulla
    • Embryo production
    • Gene expression
    • Oocyte maturation

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