TY - JOUR
T1 - From Peptide Masses to Pregnancy Maintenance
T2 - A Comprehensive Proteomic Analysis of The Early Equine Embryo Secretome, Blastocoel Fluid and Capsule
AU - Swegen, Aleona
AU - Grupen, Christopher Gerald
AU - Gibb, Zamira
AU - Baker, Mark
AU - de Ruijter - Villani, Marta
AU - Smith, Nathan Druery
AU - Stout, Tom
AU - Aitken, R John
N1 - This article is protected by copyright. All rights reserved.
PY - 2017/8/7
Y1 - 2017/8/7
N2 - Early pregnancy in the mare is a poorly understood, high risk period during which the embryo communicates its presence to the maternal endometrium. Remarkably, the maternal recognition of pregnancy signal is unknown in the horse. This study aimed to profile the proteins secreted by equine blastocysts into their immediate environment, along with proteins contained in the blastocoel and within the acellular embryo capsule. Embryos were recovered on day 8 after ovulation and cultured for 48 hours. Secretomes of day 9 and day 10 embryos were analyzed by LC-MS/MS and supported by analysis of blastocoel fluid and embryo capsule. Analyses revealed 72 (24 h) and 97 (48 h) unique protein IDs in the embryo secretome, 732 protein IDs in blastocoel fluid, and 11 proteins IDs in the embryo capsule. Novel findings of interest include secretion of a pregnancy specific proteinase (PAG) by the equine embryo at day 10, along with detection of a prostaglandin receptor inhibiting protein (PTGFRN) and a progesterone potentiating factor (FKBP4) in blastocoel fluid. This is the first comprehensive proteomic analysis of the equine embryo secretome, and provides new insights into the unique physiology of early pregnancy in this species. This article is protected by copyright. All rights reserved.
AB - Early pregnancy in the mare is a poorly understood, high risk period during which the embryo communicates its presence to the maternal endometrium. Remarkably, the maternal recognition of pregnancy signal is unknown in the horse. This study aimed to profile the proteins secreted by equine blastocysts into their immediate environment, along with proteins contained in the blastocoel and within the acellular embryo capsule. Embryos were recovered on day 8 after ovulation and cultured for 48 hours. Secretomes of day 9 and day 10 embryos were analyzed by LC-MS/MS and supported by analysis of blastocoel fluid and embryo capsule. Analyses revealed 72 (24 h) and 97 (48 h) unique protein IDs in the embryo secretome, 732 protein IDs in blastocoel fluid, and 11 proteins IDs in the embryo capsule. Novel findings of interest include secretion of a pregnancy specific proteinase (PAG) by the equine embryo at day 10, along with detection of a prostaglandin receptor inhibiting protein (PTGFRN) and a progesterone potentiating factor (FKBP4) in blastocoel fluid. This is the first comprehensive proteomic analysis of the equine embryo secretome, and provides new insights into the unique physiology of early pregnancy in this species. This article is protected by copyright. All rights reserved.
U2 - 10.1002/pmic.201600433
DO - 10.1002/pmic.201600433
M3 - Article
C2 - 28782881
SN - 1615-9853
VL - 17
JO - Proteomics
JF - Proteomics
IS - 17-18
M1 - 1600433
ER -