Follicular Dendritic Cell Isolation and Loading of Immune Complexes.

BA Heesters; der Poel CE Van; MC Carroll

doi:10.1007/978-1-4939-7095-7_9

Follicular Dendritic Cell Isolation and Loading of Immune Complexes.

BA Heesters, der Poel CE Van, MC Carroll

Harvard Medical School

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.

Original language	English
Title of host publication	Germinal Centers
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Pages	105–112
Edition	1
ISBN (Electronic)	978-1-4939-7095-7
ISBN (Print)	978-1-4939-7094-0, 978-1-4939-8383-4
DOIs	https://doi.org/10.1007/978-1-4939-7095-7_9
Publication status	Published - 8 Jun 2017
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Publisher	Humana
Volume	1623
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-4939-7095-7_9

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title = "Follicular Dendritic Cell Isolation and Loading of Immune Complexes.",

abstract = "Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.",

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AB - Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.

UR - http://europepmc.org/abstract/med/28589351

U2 - 10.1007/978-1-4939-7095-7_9

DO - 10.1007/978-1-4939-7095-7_9

M3 - Chapter

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SN - 978-1-4939-8383-4

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SP - 105

EP - 112

BT - Germinal Centers

PB - Humana Press

ER -

Follicular Dendritic Cell Isolation and Loading of Immune Complexes.

Abstract

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