Fluorescent-Probe Characterization for Pore-Space Mapping with Single-Particle Tracking

Rafael Mayorga González; J J Erik Maris; Marita Wagner; Yadolah Ganjkhanlou; Johan G Bomer; Maximilian J Werny; Freddy T Rabouw; Bert M Weckhuysen; Mathieu Odijk; Florian Meirer

doi:10.1002/anie.202314528

Fluorescent-Probe Characterization for Pore-Space Mapping with Single-Particle Tracking

Rafael Mayorga González, J J Erik Maris, Marita Wagner, Yadolah Ganjkhanlou, Johan G Bomer, Maximilian J Werny, Freddy T Rabouw, Bert M Weckhuysen, Mathieu Odijk, Florian Meirer^*

^*Corresponding author for this work

Sub Inorganic Chemistry and Catalysis

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Porous solids often contain complex pore networks with pores of various sizes. Tracking individual fluorescent probes as they diffuse through porous materials can be used to characterize pore networks at tens of nanometers resolution. However, understanding the motion behavior of fluorescent probes in confinement is crucial to reliably derive pore network properties. Here, we introduce well-defined lithography-made model pores developed to study probe behavior in confinement. We investigated the influence of probe-host interactions on diffusion and trapping of confined single-emitter quantum-dot probes. Using the pH-responsiveness of the probes, we were able to largely suppress trapping at the pore walls. This enabled us to define experimental conditions for mapping of the accessible pore space of a one-dimensional pore array as well as a real-life polymerization-catalyst-support particle.

Original language	English
Article number	e202314528
Journal	Angewandte Chemie-International Edition
Volume	63
Issue number	4
Early online date	1 Dec 2023
DOIs	https://doi.org/10.1002/anie.202314528
Publication status	Published - 22 Jan 2024

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

Funding

We would like to thank Relinde J. A. van Dijk‐Moes (Utrecht University, UU) for transmission electron microscopy on the QDs and Arnout Imhof (UU) for fruitful discussions. This work was supported by the Netherlands Center for Multiscale Catalytic Energy Conversion (MCEC), an NWO Gravitation Program funded by the Ministry of Education, Culture and Science of the Government of the Netherlands. F.M. also acknowledges funding from the Netherlands Organization for Scientific Research (NWO) VIDI Grant (No. 723.015.007).

Funders	Funder number
MCEC
Netherlands Center for Multiscale Catalytic Energy Conversion
Ministerie van onderwijs, cultuur en wetenschap
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	723.015.007

Keywords

Diffusion
Nanofluidics
Porosity Characterization
Single Particle Tracking
Single-Molecule Localization Microscopy

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10.1002/anie.202314528Licence: CC BY

Angew Chem Int Ed - 2023 - González - Fluorescent‐Probe Characterization for Pore‐Space Mapping with Single‐ParticleFinal published version, 2.73 MBLicence: CC BY

Cite this

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title = "Fluorescent-Probe Characterization for Pore-Space Mapping with Single-Particle Tracking",

abstract = "Porous solids often contain complex pore networks with pores of various sizes. Tracking individual fluorescent probes as they diffuse through porous materials can be used to characterize pore networks at tens of nanometers resolution. However, understanding the motion behavior of fluorescent probes in confinement is crucial to reliably derive pore network properties. Here, we introduce well-defined lithography-made model pores developed to study probe behavior in confinement. We investigated the influence of probe-host interactions on diffusion and trapping of confined single-emitter quantum-dot probes. Using the pH-responsiveness of the probes, we were able to largely suppress trapping at the pore walls. This enabled us to define experimental conditions for mapping of the accessible pore space of a one-dimensional pore array as well as a real-life polymerization-catalyst-support particle.",

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AU - González, Rafael Mayorga

AU - Maris, J J Erik

AU - Wagner, Marita

AU - Ganjkhanlou, Yadolah

AU - Bomer, Johan G

AU - Werny, Maximilian J

AU - Rabouw, Freddy T

AU - Weckhuysen, Bert M

AU - Odijk, Mathieu

AU - Meirer, Florian

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AB - Porous solids often contain complex pore networks with pores of various sizes. Tracking individual fluorescent probes as they diffuse through porous materials can be used to characterize pore networks at tens of nanometers resolution. However, understanding the motion behavior of fluorescent probes in confinement is crucial to reliably derive pore network properties. Here, we introduce well-defined lithography-made model pores developed to study probe behavior in confinement. We investigated the influence of probe-host interactions on diffusion and trapping of confined single-emitter quantum-dot probes. Using the pH-responsiveness of the probes, we were able to largely suppress trapping at the pore walls. This enabled us to define experimental conditions for mapping of the accessible pore space of a one-dimensional pore array as well as a real-life polymerization-catalyst-support particle.

KW - Diffusion

KW - Nanofluidics

KW - Porosity Characterization

KW - Single Particle Tracking

KW - Single-Molecule Localization Microscopy

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M3 - Article

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JO - Angewandte Chemie-International Edition

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ER -

Fluorescent-Probe Characterization for Pore-Space Mapping with Single-Particle Tracking

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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