Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

F. Ariese, A.N. Bader, C. de Gooijer

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Fluorescence line-narrowing spectroscopy (FLNS) is a cryogenic technique that can be used to obtain high-resolution fluorescence spectra. The technique has mainly been used for fingerprint identification. However, this review focuses on the trend to use FLNS for probing purposes: obtaining information on interactions with the local environment or determining conformations. We briefly discuss the basics of FLNS, solute-matrix interactions and important instrumental aspects. We describe examples from various bioanalytical fields: DNA adducts of the aromatic carcinogen benzo[a]pyrene (BP); binding of BP metabolites to monoclonal antibodies or the estrogen receptor; FLNS studies of porphyrin-containing proteins; and, photosynthetic systems. As an illustration from the field of environmental chemistry, we discuss the use of luminescence line-narrowing spectroscopy - a special mode of FLNS - to study the complexation of lanthanides to humic substances.
Original languageUndefined/Unknown
Pages (from-to)127-138
Number of pages12
JournalTrends in Analytical Chemistry
Issue number2
Publication statusPublished - 2008

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