Fish-meal diet enriched with omega-3 PUFA and treatment of canine chronic enteropathies

Edgar Corneille Ontsouka; Iwan Anton Burgener; Nicole Luckschander-Zeller; Juerg Walter Blum; Christiane Albrecht

doi:10.1002/ejlt.201100343

Fish-meal diet enriched with omega-3 PUFA and treatment of canine chronic enteropathies

Edgar Corneille Ontsouka^*, Iwan Anton Burgener, Nicole Luckschander-Zeller, Juerg Walter Blum, Christiane Albrecht

^*Corresponding author for this work

University of Bern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The effects of a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA on intestinal inflammatory activity and expression of genes active in fatty acid (FA) uptake were tested in the duodenum of dogs with food responsive diarrhea (FRD; n?=?14) and inflammatory bowel disease (IBD; n?=?7). The clinical outcome was estimated by monitoring the canine IBD activity index (CIBDAI) before and after treatment. Dogs were treated with the diet alone (FRD) or the diet in combination with immunosuppressants (IBD). The duodenal mRNA levels of FA translocase, FA transport protein-1,-3,-4,-6, long chain acyl coenzyme synthetase-1,-4,-5,-6, liver- and intestinal-FA binding proteins, interleukin-1 beta (IL)-1 beta and -6, and tumor necrosis factor-a were measured by quantitative PCR. The CIBDAI significantly decreased after treatment in all dogs. The mRNA levels of target genes were associated both with disease phenotype and dietary treatment. Significantly different expression patterns were found for IL-1 beta, FA translocase, intestinal-FA binding protein, FA transport proteins-1,-3,-6, and long chain acyl coenzyme synthetase-5,-6. In conclusion, the mRNA levels of some of the genes involved in duodenal FA uptake may be altered by a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA. This may be beneficial for the treatment of canine chronic enteropathies, particularly FRD.

Original language	English
Pages (from-to)	412-422
Number of pages	11
Journal	European Journal of Lipid Science and Technology
Volume	114
Issue number	4
DOIs	https://doi.org/10.1002/ejlt.201100343
Publication status	Published - Apr 2012

Funding

This study was supported by grants from the SwissLife Jubilaumsstiftung, the Novartis Foundation, and by the Department of Clinical Veterinary Medicine of the Vetsuisse Faculty. We thank Maria Feher at the Institute of Biochemistry and Molecular Medicine for mRNA isolation. We also thank Biomill SA (Granges-Marnand, Switzerland) for preparing the diets and particularly Dr. Dorothee Isler for preparing the elimination diet.

Keywords

Canine inflammatory bowel disease index activity
Fatty acid transporters
Intestinal bowel disease
Omega-6 and omega-3 fatty acids
INFLAMMATORY-BOWEL-DISEASE
ACID-BINDING PROTEINS
POLYUNSATURATED FATTY-ACIDS
MESSENGER-RNA EXPRESSION
ACYL-COA SYNTHETASE
CHOLESTEROL UPTAKE
CELLULAR UPTAKE
DOGS
INTESTINE
METABOLISM

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/ejlt.201100343

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title = "Fish-meal diet enriched with omega-3 PUFA and treatment of canine chronic enteropathies",

abstract = "The effects of a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA on intestinal inflammatory activity and expression of genes active in fatty acid (FA) uptake were tested in the duodenum of dogs with food responsive diarrhea (FRD; n?=?14) and inflammatory bowel disease (IBD; n?=?7). The clinical outcome was estimated by monitoring the canine IBD activity index (CIBDAI) before and after treatment. Dogs were treated with the diet alone (FRD) or the diet in combination with immunosuppressants (IBD). The duodenal mRNA levels of FA translocase, FA transport protein-1,-3,-4,-6, long chain acyl coenzyme synthetase-1,-4,-5,-6, liver- and intestinal-FA binding proteins, interleukin-1 beta (IL)-1 beta and -6, and tumor necrosis factor-a were measured by quantitative PCR. The CIBDAI significantly decreased after treatment in all dogs. The mRNA levels of target genes were associated both with disease phenotype and dietary treatment. Significantly different expression patterns were found for IL-1 beta, FA translocase, intestinal-FA binding protein, FA transport proteins-1,-3,-6, and long chain acyl coenzyme synthetase-5,-6. In conclusion, the mRNA levels of some of the genes involved in duodenal FA uptake may be altered by a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA. This may be beneficial for the treatment of canine chronic enteropathies, particularly FRD.",

keywords = "Canine inflammatory bowel disease index activity, Fatty acid transporters, Intestinal bowel disease, Omega-6 and omega-3 fatty acids, INFLAMMATORY-BOWEL-DISEASE, ACID-BINDING PROTEINS, POLYUNSATURATED FATTY-ACIDS, MESSENGER-RNA EXPRESSION, ACYL-COA SYNTHETASE, CHOLESTEROL UPTAKE, CELLULAR UPTAKE, DOGS, INTESTINE, METABOLISM",

author = "Ontsouka, {Edgar Corneille} and Burgener, {Iwan Anton} and Nicole Luckschander-Zeller and Blum, {Juerg Walter} and Christiane Albrecht",

year = "2012",

month = apr,

doi = "10.1002/ejlt.201100343",

language = "English",

volume = "114",

pages = "412--422",

journal = "European Journal of Lipid Science and Technology",

issn = "1438-7697",

publisher = "Wiley-VCH Verlag",

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}

TY - JOUR

T1 - Fish-meal diet enriched with omega-3 PUFA and treatment of canine chronic enteropathies

AU - Ontsouka, Edgar Corneille

AU - Burgener, Iwan Anton

AU - Luckschander-Zeller, Nicole

AU - Blum, Juerg Walter

AU - Albrecht, Christiane

PY - 2012/4

Y1 - 2012/4

N2 - The effects of a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA on intestinal inflammatory activity and expression of genes active in fatty acid (FA) uptake were tested in the duodenum of dogs with food responsive diarrhea (FRD; n?=?14) and inflammatory bowel disease (IBD; n?=?7). The clinical outcome was estimated by monitoring the canine IBD activity index (CIBDAI) before and after treatment. Dogs were treated with the diet alone (FRD) or the diet in combination with immunosuppressants (IBD). The duodenal mRNA levels of FA translocase, FA transport protein-1,-3,-4,-6, long chain acyl coenzyme synthetase-1,-4,-5,-6, liver- and intestinal-FA binding proteins, interleukin-1 beta (IL)-1 beta and -6, and tumor necrosis factor-a were measured by quantitative PCR. The CIBDAI significantly decreased after treatment in all dogs. The mRNA levels of target genes were associated both with disease phenotype and dietary treatment. Significantly different expression patterns were found for IL-1 beta, FA translocase, intestinal-FA binding protein, FA transport proteins-1,-3,-6, and long chain acyl coenzyme synthetase-5,-6. In conclusion, the mRNA levels of some of the genes involved in duodenal FA uptake may be altered by a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA. This may be beneficial for the treatment of canine chronic enteropathies, particularly FRD.

AB - The effects of a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA on intestinal inflammatory activity and expression of genes active in fatty acid (FA) uptake were tested in the duodenum of dogs with food responsive diarrhea (FRD; n?=?14) and inflammatory bowel disease (IBD; n?=?7). The clinical outcome was estimated by monitoring the canine IBD activity index (CIBDAI) before and after treatment. Dogs were treated with the diet alone (FRD) or the diet in combination with immunosuppressants (IBD). The duodenal mRNA levels of FA translocase, FA transport protein-1,-3,-4,-6, long chain acyl coenzyme synthetase-1,-4,-5,-6, liver- and intestinal-FA binding proteins, interleukin-1 beta (IL)-1 beta and -6, and tumor necrosis factor-a were measured by quantitative PCR. The CIBDAI significantly decreased after treatment in all dogs. The mRNA levels of target genes were associated both with disease phenotype and dietary treatment. Significantly different expression patterns were found for IL-1 beta, FA translocase, intestinal-FA binding protein, FA transport proteins-1,-3,-6, and long chain acyl coenzyme synthetase-5,-6. In conclusion, the mRNA levels of some of the genes involved in duodenal FA uptake may be altered by a fish-meal- and potato-protein-based diet enriched with omega-3 PUFA. This may be beneficial for the treatment of canine chronic enteropathies, particularly FRD.

KW - Canine inflammatory bowel disease index activity

KW - Fatty acid transporters

KW - Intestinal bowel disease

KW - Omega-6 and omega-3 fatty acids

KW - INFLAMMATORY-BOWEL-DISEASE

KW - ACID-BINDING PROTEINS

KW - POLYUNSATURATED FATTY-ACIDS

KW - MESSENGER-RNA EXPRESSION

KW - ACYL-COA SYNTHETASE

KW - CHOLESTEROL UPTAKE

KW - CELLULAR UPTAKE

KW - DOGS

KW - INTESTINE

KW - METABOLISM

U2 - 10.1002/ejlt.201100343

DO - 10.1002/ejlt.201100343

M3 - Article

SN - 1438-7697

VL - 114

SP - 412

EP - 422

JO - European Journal of Lipid Science and Technology

JF - European Journal of Lipid Science and Technology

IS - 4

ER -

Fish-meal diet enriched with omega-3 PUFA and treatment of canine chronic enteropathies

Abstract

Funding

Keywords

UN SDGs

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