Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

E.M.D.L. van der Heijden; A.O. Jenkins; D. Cooper; V.P.M.G. Rutten; A.L. Michel

Abstract

The African buffalo (Syncerus caffer) is considered the most important maintenance host of BTB (bovine tuberculosis) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the SICTT (single intradermal comparative tuberculin test). As an alternative, the BOVIGAM® 1G, an IFN-γ (interferon-gamma) release assay, is frequently used. The objective of this study was to compare the test performance of CMI- (cell-mediated immunity) and HI- (humoral immunity) based assays for the detection of M. bovis infections in buffaloes for fitness of the test purpose. Buffaloes were sampled during the annual BTB testing in the Hluhluwe-iMfolozi-Park (KwaZulu-Natal, South Africa) during June 2013. A total of 58 animals were subjected to the SICTT, 17 of these tested positive and 1 showed an inconclusive reaction. CMI based assays (B1G (BOVIGAM® 1G) and B2G (BOVIGAM® 2G)) as well as a serological assay (IDEXX TB ELISA) were used to further investigate immune responsiveness of these 18 animals and 40 SICTT negative animals. Seventeen SICTT positive and suspect buffaloes were slaughtered and a PM (post-mortem) examination was conducted. Test results of individual assays were compared with serial and parallel test interpretation. Post-mortem examination was used to confirm BTB. Lesions characteristic of BTB were found in 9 animals (52.9%). The B1G assay showed the highest individual sensitivity (85.7%) followed by the B2G assay (75%) and the IDEXX TB ELISA (43.8%). Using in parallel interpretation, the combination of the SICTT and the BOVIGAM® assay or the combination of the BOVIGAM® assay and the IDEXX TB ELISA showed the highest sensitivity (100%). In conclusion, this study has shown that the BOVIGAM® IFN-γ assay has the highest test performance and when used alone or in conjunction with the IDEXX TB ELISA it is fit for the diagnosis of BTB in free-roaming African buffaloes.

Original language	English
Publication status	Published - Aug 2015
Event	The 13th annual congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine - Bloemfontein, South Africa Duration: 19 Aug 2015 → 21 Aug 2015 http://sasvepm.org/wp-content/uploads/2016/05/sasvepm2015_proceedings_version2.pdf

Conference

Conference	The 13th annual congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine
Country/Territory	South Africa
City	Bloemfontein
Period	19/08/15 → 21/08/15
Internet address	http://sasvepm.org/wp-content/uploads/2016/05/sasvepm2015_proceedings_version2.pdf

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

1 Article

Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)
van der Heijden, E. M. D. L., Jenkins, A., Cooper, D., Rutten, V. P. M. G. & Michel, A. L., 1 Jan 2016, In: Veterinary Immunology and Immunopathology. 169, p. 68-73
Research output: Contribution to journal › Article › Academic › peer-review

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van der Heijden, E. M. D. L., Jenkins, A. O., Cooper, D., Rutten, V. P. M. G., & Michel, A. L. (2015). Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer). Abstract from The 13th annual congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine, Bloemfontein, South Africa.

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title = "Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)",

abstract = "The African buffalo (Syncerus caffer) is considered the most important maintenance host of BTB (bovine tuberculosis) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the SICTT (single intradermal comparative tuberculin test). As an alternative, the BOVIGAM{\textregistered} 1G, an IFN-γ (interferon-gamma) release assay, is frequently used. The objective of this study was to compare the test performance of CMI- (cell-mediated immunity) and HI- (humoral immunity) based assays for the detection of M. bovis infections in buffaloes for fitness of the test purpose. Buffaloes were sampled during the annual BTB testing in the Hluhluwe-iMfolozi-Park (KwaZulu-Natal, South Africa) during June 2013. A total of 58 animals were subjected to the SICTT, 17 of these tested positive and 1 showed an inconclusive reaction. CMI based assays (B1G (BOVIGAM{\textregistered} 1G) and B2G (BOVIGAM{\textregistered} 2G)) as well as a serological assay (IDEXX TB ELISA) were used to further investigate immune responsiveness of these 18 animals and 40 SICTT negative animals. Seventeen SICTT positive and suspect buffaloes were slaughtered and a PM (post-mortem) examination was conducted. Test results of individual assays were compared with serial and parallel test interpretation. Post-mortem examination was used to confirm BTB. Lesions characteristic of BTB were found in 9 animals (52.9%). The B1G assay showed the highest individual sensitivity (85.7%) followed by the B2G assay (75%) and the IDEXX TB ELISA (43.8%). Using in parallel interpretation, the combination of the SICTT and the BOVIGAM{\textregistered} assay or the combination of the BOVIGAM{\textregistered} assay and the IDEXX TB ELISA showed the highest sensitivity (100%). In conclusion, this study has shown that the BOVIGAM{\textregistered} IFN-γ assay has the highest test performance and when used alone or in conjunction with the IDEXX TB ELISA it is fit for the diagnosis of BTB in free-roaming African buffaloes.",

author = "{van der Heijden}, E.M.D.L. and A.O. Jenkins and D. Cooper and V.P.M.G. Rutten and A.L. Michel",

year = "2015",

month = aug,

language = "English",

note = "The 13th annual congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine ; Conference date: 19-08-2015 Through 21-08-2015",

url = "http://sasvepm.org/wp-content/uploads/2016/05/sasvepm2015_proceedings_version2.pdf",

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Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer). / van der Heijden, E.M.D.L.; Jenkins, A.O.; Cooper, D. et al.
2015. Abstract from The 13th annual congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine, Bloemfontein, South Africa.

Research output: Contribution to conference › Abstract › Other research output

TY - CONF

T1 - Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

AU - van der Heijden, E.M.D.L.

AU - Jenkins, A.O.

AU - Cooper, D.

AU - Rutten, V.P.M.G.

AU - Michel, A.L.

PY - 2015/8

Y1 - 2015/8

N2 - The African buffalo (Syncerus caffer) is considered the most important maintenance host of BTB (bovine tuberculosis) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the SICTT (single intradermal comparative tuberculin test). As an alternative, the BOVIGAM® 1G, an IFN-γ (interferon-gamma) release assay, is frequently used. The objective of this study was to compare the test performance of CMI- (cell-mediated immunity) and HI- (humoral immunity) based assays for the detection of M. bovis infections in buffaloes for fitness of the test purpose. Buffaloes were sampled during the annual BTB testing in the Hluhluwe-iMfolozi-Park (KwaZulu-Natal, South Africa) during June 2013. A total of 58 animals were subjected to the SICTT, 17 of these tested positive and 1 showed an inconclusive reaction. CMI based assays (B1G (BOVIGAM® 1G) and B2G (BOVIGAM® 2G)) as well as a serological assay (IDEXX TB ELISA) were used to further investigate immune responsiveness of these 18 animals and 40 SICTT negative animals. Seventeen SICTT positive and suspect buffaloes were slaughtered and a PM (post-mortem) examination was conducted. Test results of individual assays were compared with serial and parallel test interpretation. Post-mortem examination was used to confirm BTB. Lesions characteristic of BTB were found in 9 animals (52.9%). The B1G assay showed the highest individual sensitivity (85.7%) followed by the B2G assay (75%) and the IDEXX TB ELISA (43.8%). Using in parallel interpretation, the combination of the SICTT and the BOVIGAM® assay or the combination of the BOVIGAM® assay and the IDEXX TB ELISA showed the highest sensitivity (100%). In conclusion, this study has shown that the BOVIGAM® IFN-γ assay has the highest test performance and when used alone or in conjunction with the IDEXX TB ELISA it is fit for the diagnosis of BTB in free-roaming African buffaloes.

AB - The African buffalo (Syncerus caffer) is considered the most important maintenance host of BTB (bovine tuberculosis) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the SICTT (single intradermal comparative tuberculin test). As an alternative, the BOVIGAM® 1G, an IFN-γ (interferon-gamma) release assay, is frequently used. The objective of this study was to compare the test performance of CMI- (cell-mediated immunity) and HI- (humoral immunity) based assays for the detection of M. bovis infections in buffaloes for fitness of the test purpose. Buffaloes were sampled during the annual BTB testing in the Hluhluwe-iMfolozi-Park (KwaZulu-Natal, South Africa) during June 2013. A total of 58 animals were subjected to the SICTT, 17 of these tested positive and 1 showed an inconclusive reaction. CMI based assays (B1G (BOVIGAM® 1G) and B2G (BOVIGAM® 2G)) as well as a serological assay (IDEXX TB ELISA) were used to further investigate immune responsiveness of these 18 animals and 40 SICTT negative animals. Seventeen SICTT positive and suspect buffaloes were slaughtered and a PM (post-mortem) examination was conducted. Test results of individual assays were compared with serial and parallel test interpretation. Post-mortem examination was used to confirm BTB. Lesions characteristic of BTB were found in 9 animals (52.9%). The B1G assay showed the highest individual sensitivity (85.7%) followed by the B2G assay (75%) and the IDEXX TB ELISA (43.8%). Using in parallel interpretation, the combination of the SICTT and the BOVIGAM® assay or the combination of the BOVIGAM® assay and the IDEXX TB ELISA showed the highest sensitivity (100%). In conclusion, this study has shown that the BOVIGAM® IFN-γ assay has the highest test performance and when used alone or in conjunction with the IDEXX TB ELISA it is fit for the diagnosis of BTB in free-roaming African buffaloes.

M3 - Abstract

T2 - The 13th annual congress of the Southern African Society for Veterinary Epidemiology and Preventive Medicine

Y2 - 19 August 2015 through 21 August 2015

ER -

Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

Abstract

Conference

UN SDGs

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Research output

Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

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