Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts

H Aardema; I Bertijn; HTA van Tol; A Rijneveld; JCM Vernooij; B M Gadella; PLAM Vos

doi:10.3389/fcell.2022.837405

Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts

H Aardema
, I Bertijn
, HTA van Tol
, A Rijneveld
, JCM Vernooij
, B M Gadella
, PLAM Vos

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C 18:0) or unsaturated oleic (C 18:1) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C 18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C 18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C 18:0 condition rates were higher (43.2%) when compared to C 18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts.

Original language	English
Article number	837405
Pages (from-to)	1-14
Journal	Frontiers in Cell and Developmental Biology
Volume	10
DOIs	https://doi.org/10.3389/fcell.2022.837405
Publication status	Published - 9 Mar 2022

Bibliographical note

Funding Information:
Imaging of the embryos was performed at the Centre for Cellular Imaging of the faculty of Veterinary Medicine of Utrecht University (the Netherlands) under the supervison of R. Wubbolts and Ing. E. van?t Veld. The LD540 probe was produced and supplied as personal gift by Professor C. Thiele (Univ. of Bonn, Germany). We thank breeding company CRV for the generously provided frozen embryos.

Publisher Copyright:
Copyright © 2022 Aardema, Bertijn, van Tol, Rijneveld, Vernooij, Gadella and Vos.

Keywords

apoptose
blastocyst
cryopreservation
cryosurvival
embryo
free fatty acid
lipid
slow freezing

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title = "Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts",

abstract = " In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C 18:0) or unsaturated oleic (C 18:1) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C 18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4\%, respectively) comparable with in vivo blastocysts (68\%), in contrast to the poor cryosurvival of C 18:0 exposed embryos (17.6\%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C 18:0 condition rates were higher (43.2\%) when compared to C 18:1 (26.0\%) and FAF SOF conditions (26.5\%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts. ",

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author = "H Aardema and I Bertijn and \{van Tol\}, HTA and A Rijneveld and JCM Vernooij and Gadella, \{B M\} and PLAM Vos",

note = "Funding Information: Imaging of the embryos was performed at the Centre for Cellular Imaging of the faculty of Veterinary Medicine of Utrecht University (the Netherlands) under the supervison of R. Wubbolts and Ing. E. van?t Veld. The LD540 probe was produced and supplied as personal gift by Professor C. Thiele (Univ. of Bonn, Germany). We thank breeding company CRV for the generously provided frozen embryos. Publisher Copyright: Copyright {\textcopyright} 2022 Aardema, Bertijn, van Tol, Rijneveld, Vernooij, Gadella and Vos.",

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AU - Bertijn, I

AU - van Tol, HTA

AU - Rijneveld, A

AU - Vernooij, JCM

AU - Gadella, B M

AU - Vos, PLAM

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PY - 2022/3/9

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N2 - In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C 18:0) or unsaturated oleic (C 18:1) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C 18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C 18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C 18:0 condition rates were higher (43.2%) when compared to C 18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts.

AB - In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C 18:0) or unsaturated oleic (C 18:1) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C 18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C 18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C 18:0 condition rates were higher (43.2%) when compared to C 18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts.

KW - apoptose

KW - blastocyst

KW - cryopreservation

KW - cryosurvival

KW - embryo

KW - free fatty acid

KW - lipid

KW - slow freezing

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U2 - 10.3389/fcell.2022.837405

DO - 10.3389/fcell.2022.837405

M3 - Article

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SN - 2296-634X

VL - 10

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JO - Frontiers in Cell and Developmental Biology

JF - Frontiers in Cell and Developmental Biology

M1 - 837405

ER -

Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts

Abstract

Bibliographical note

Keywords

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