TY - JOUR
T1 - Factor VIII interacts with the endocytic receptor low-density lipoprotein receptor-related protein 1 via an extended surface comprising "hot-spot" lysine residues
AU - Van Den Biggelaar, Maartje
AU - Madsen, Jesper J.
AU - Faber, Johan H.
AU - Zuurveld, Marleen G.
AU - Van Der Zwaan, Carmen
AU - Olsen, Ole H.
AU - Stennicke, Henning R.
AU - Mertens, Koen
AU - Meijer, Alexander B.
PY - 2015/7/3
Y1 - 2015/7/3
N2 - Background: It is unclear how the LDL receptor family binds large protein ligands. Results: HDX and lysine scanning identified factor (F)VIII regions and specific lysine residues binding low-density lipoprotein receptor-related protein 1 (LRP1). Conclusion: FVIII-LRP1 interaction involves multiple "hot-spot" lysine residues in the A3C1 domains. Significance: Our study sheds light on interactions of complex ligands with the LDL receptor family.
AB - Background: It is unclear how the LDL receptor family binds large protein ligands. Results: HDX and lysine scanning identified factor (F)VIII regions and specific lysine residues binding low-density lipoprotein receptor-related protein 1 (LRP1). Conclusion: FVIII-LRP1 interaction involves multiple "hot-spot" lysine residues in the A3C1 domains. Significance: Our study sheds light on interactions of complex ligands with the LDL receptor family.
UR - https://www.scopus.com/pages/publications/84936805995
U2 - 10.1074/jbc.M115.650911
DO - 10.1074/jbc.M115.650911
M3 - Article
AN - SCOPUS:84936805995
SN - 0021-9258
VL - 290
SP - 16463
EP - 16476
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 27
ER -