Facile electrochemical affinity measurements of small and large molecules

Pouya Zaree; Ilhan Tomris; Sander D. de Vos; Roosmarijn van der Woude; Frits M. Flesch; Robertus J.M. Klein Gebbink; Robert P. de Vries; Roland J. Pieters

doi:10.1039/d3ra01029e

Facile electrochemical affinity measurements of small and large molecules

Pouya Zaree, Ilhan Tomris, Sander D. de Vos, Roosmarijn van der Woude, Frits M. Flesch, Robertus J.M. Klein Gebbink, Robert P. de Vries, Roland J. Pieters^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

Original language	English
Pages (from-to)	9756-9760
Number of pages	5
Journal	RSC Advances
Volume	13
Issue number	14
DOIs	https://doi.org/10.1039/d3ra01029e
Publication status	Published - 27 Mar 2023

Bibliographical note

Publisher Copyright:
© 2023 The Royal Society of Chemistry.

Funding

We thank professor R. Sanders, (Amsterdam Medical Center), for making cDNAs encoding codon-optimized open reading frames of full-length SARS-CoV-2, B.1.1.7 and B.1.351 spikes available.

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title = "Facile electrochemical affinity measurements of small and large molecules",

abstract = "A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.",

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AU - Tomris, Ilhan

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AU - Flesch, Frits M.

AU - Klein Gebbink, Robertus J.M.

AU - de Vries, Robert P.

AU - Pieters, Roland J.

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AB - A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

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ER -

Facile electrochemical affinity measurements of small and large molecules

Abstract

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