Facile electrochemical affinity measurements of small and large molecules

Pouya Zaree; Ilhan Tomris; Sander D. de Vos; Roosmarijn van der Woude; Frits M. Flesch; Robertus J.M. Klein Gebbink; Robert P. de Vries; Roland J. Pieters

doi:10.1039/d3ra01029e

Facile electrochemical affinity measurements of small and large molecules

Pouya Zaree, Ilhan Tomris, Sander D. de Vos, Roosmarijn van der Woude, Frits M. Flesch, Robertus J.M. Klein Gebbink, Robert P. de Vries, Roland J. Pieters^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

Original language	English
Pages (from-to)	9756-9760
Number of pages	5
Journal	RSC Advances
Volume	13
Issue number	14
DOIs	https://doi.org/10.1039/d3ra01029e
Publication status	Published - 27 Mar 2023

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title = "Facile electrochemical affinity measurements of small and large molecules",

abstract = "A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.",

author = "Pouya Zaree and Ilhan Tomris and {de Vos}, {Sander D.} and {van der Woude}, Roosmarijn and Flesch, {Frits M.} and {Klein Gebbink}, {Robertus J.M.} and {de Vries}, {Robert P.} and Pieters, {Roland J.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Royal Society of Chemistry.",

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doi = "10.1039/d3ra01029e",

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T1 - Facile electrochemical affinity measurements of small and large molecules

AU - Zaree, Pouya

AU - Tomris, Ilhan

AU - de Vos, Sander D.

AU - van der Woude, Roosmarijn

AU - Flesch, Frits M.

AU - Klein Gebbink, Robertus J.M.

AU - de Vries, Robert P.

AU - Pieters, Roland J.

PY - 2023/3/27

Y1 - 2023/3/27

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AB - A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

UR - http://www.scopus.com/inward/record.url?scp=85152213436&partnerID=8YFLogxK

U2 - 10.1039/d3ra01029e

DO - 10.1039/d3ra01029e

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VL - 13

SP - 9756

EP - 9760

JO - RSC Advances

JF - RSC Advances

IS - 14

ER -

Facile electrochemical affinity measurements of small and large molecules

Abstract

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