Extraction of SV40 T-antigen from lysates of transformed cells

J. A. Lenstra; R. A M M Smits; H. Bloemendal

doi:10.1016/0014-5793(84)80221-5

Extraction of SV40 T-antigen from lysates of transformed cells

J. A. Lenstra^*, R. A M M Smits, H. Bloemendal

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca²⁺ at low temperature, conditions that also cause a specific depolymerization of microtubules.

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	FEBS Letters
Volume	168
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80221-5
Publication status	Published - 12 Mar 1984

Keywords

T-antigen
Transformed hamster lens cell

Access to Document

10.1016/0014-5793(84)80221-5

Cite this

@article{5ed358f0c7eb43728126e822e49eb91f,

title = "Extraction of SV40 T-antigen from lysates of transformed cells",

abstract = "Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca2+ at low temperature, conditions that also cause a specific depolymerization of microtubules.",

keywords = "T-antigen, Transformed hamster lens cell",

author = "Lenstra, {J. A.} and Smits, {R. A M M} and H. Bloemendal",

year = "1984",

month = mar,

day = "12",

doi = "10.1016/0014-5793(84)80221-5",

language = "English",

volume = "168",

pages = "129--133",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Extraction of SV40 T-antigen from lysates of transformed cells

AU - Lenstra, J. A.

AU - Smits, R. A M M

AU - Bloemendal, H.

PY - 1984/3/12

Y1 - 1984/3/12

N2 - Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca2+ at low temperature, conditions that also cause a specific depolymerization of microtubules.

AB - Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca2+ at low temperature, conditions that also cause a specific depolymerization of microtubules.

KW - T-antigen

KW - Transformed hamster lens cell

UR - http://www.scopus.com/inward/record.url?scp=0021760918&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(84)80221-5

DO - 10.1016/0014-5793(84)80221-5

M3 - Article

C2 - 6323217

AN - SCOPUS:0021760918

SN - 0014-5793

VL - 168

SP - 129

EP - 133

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Extraction of SV40 T-antigen from lysates of transformed cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this