Expression of Toll-like receptor 2 in duodenal biopsies from dogs with inflammatory bowel disease is associated with severity of disease

L. A. McMahon; A. K. House; B. Catchpole; J. Elson-Riggins; A. Riddle; K. Smith; D. Werling; I. A. Burgener; K. Allenspach

doi:10.1016/j.vetimm.2009.11.012

Expression of Toll-like receptor 2 in duodenal biopsies from dogs with inflammatory bowel disease is associated with severity of disease

L. A. McMahon^*, A. K. House, B. Catchpole, J. Elson-Riggins, A. Riddle, K. Smith, D. Werling, I. A. Burgener, K. Allenspach

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

There is growing evidence that aberrant innate immune responses towards the bacterial flora of the gut play a role in the pathogenesis of canine inflammatory bowel disease (IBD). Toll-like receptors (TLR) play an important role as primary sensors of invading pathogens and have gained significant attention in human IBD as differential expression and polymorphisms of certain TLR have been shown to occur in ulcerative colitis (DC) and Crohn's disease (CD).

The aim of the current study was to evaluate the expression of two TLR important for recognition of commensals in the gut. TLR2 and TLR4 mRNA expression in duodenal biopsies from dogs with IBD was measured and correlated with clinical and histological disease severity. Endoscopic duodenal biopsies from 20 clinical cases and 7 healthy control dogs were used to extract mRNA. TLR2 and TLR4 mRNA expression was assessed using quantitative real-time PCR. TLR2 mRNA expression was significantly increased in the IBD dogs compared to controls, whereas TLR4 mRNA expression was similar in IBD and control cases. In addition, TLR2 mRNA expression was mildly correlated with clinical severity of disease, however, there was no correlation between TLR2 expression and histological severity of disease. (C) 2009 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	158-163
Number of pages	6
Journal	Veterinary Immunology and Immunopathology
Volume	135
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetimm.2009.11.012
Publication status	Published - 15 May 2010

Funding

The authors wish to thank owners of dogs with IBD, clinicians and nurses from the Queen Mother Hospital for Animals, Royal Veterinary College, Roz Douthwaite for help with preparation of the histopathology samples and the Clinical Investigation Centre of the Royal Veterinary College. This work was supported by a grant form CEVA Sante Animate, France, an internal grant form the Royal Veterinary College, London, a grant from the Comparative Gastroenterology Society, a grant from Petplan and a Wellcome Trust equipment grant.

Keywords

Toll-like receptors
Dog
Inflammatory bowel disease
CHRONIC ENTEROPATHIES
TLR4
COLITIS
HEALTH
CATS
GUT

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetimm.2009.11.012

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McMahon, L. A., House, A. K., Catchpole, B., Elson-Riggins, J., Riddle, A., Smith, K., Werling, D., Burgener, I. A., & Allenspach, K. (2010). Expression of Toll-like receptor 2 in duodenal biopsies from dogs with inflammatory bowel disease is associated with severity of disease. Veterinary Immunology and Immunopathology, 135(1-2), 158-163. https://doi.org/10.1016/j.vetimm.2009.11.012

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abstract = "There is growing evidence that aberrant innate immune responses towards the bacterial flora of the gut play a role in the pathogenesis of canine inflammatory bowel disease (IBD). Toll-like receptors (TLR) play an important role as primary sensors of invading pathogens and have gained significant attention in human IBD as differential expression and polymorphisms of certain TLR have been shown to occur in ulcerative colitis (DC) and Crohn's disease (CD).The aim of the current study was to evaluate the expression of two TLR important for recognition of commensals in the gut. TLR2 and TLR4 mRNA expression in duodenal biopsies from dogs with IBD was measured and correlated with clinical and histological disease severity. Endoscopic duodenal biopsies from 20 clinical cases and 7 healthy control dogs were used to extract mRNA. TLR2 and TLR4 mRNA expression was assessed using quantitative real-time PCR. TLR2 mRNA expression was significantly increased in the IBD dogs compared to controls, whereas TLR4 mRNA expression was similar in IBD and control cases. In addition, TLR2 mRNA expression was mildly correlated with clinical severity of disease, however, there was no correlation between TLR2 expression and histological severity of disease. (C) 2009 Elsevier B.V. All rights reserved.",

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McMahon, LA, House, AK, Catchpole, B, Elson-Riggins, J, Riddle, A, Smith, K, Werling, D, Burgener, IA & Allenspach, K 2010, 'Expression of Toll-like receptor 2 in duodenal biopsies from dogs with inflammatory bowel disease is associated with severity of disease', Veterinary Immunology and Immunopathology, vol. 135, no. 1-2, pp. 158-163. https://doi.org/10.1016/j.vetimm.2009.11.012

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AU - McMahon, L. A.

AU - House, A. K.

AU - Catchpole, B.

AU - Elson-Riggins, J.

AU - Riddle, A.

AU - Smith, K.

AU - Werling, D.

AU - Burgener, I. A.

AU - Allenspach, K.

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N2 - There is growing evidence that aberrant innate immune responses towards the bacterial flora of the gut play a role in the pathogenesis of canine inflammatory bowel disease (IBD). Toll-like receptors (TLR) play an important role as primary sensors of invading pathogens and have gained significant attention in human IBD as differential expression and polymorphisms of certain TLR have been shown to occur in ulcerative colitis (DC) and Crohn's disease (CD).The aim of the current study was to evaluate the expression of two TLR important for recognition of commensals in the gut. TLR2 and TLR4 mRNA expression in duodenal biopsies from dogs with IBD was measured and correlated with clinical and histological disease severity. Endoscopic duodenal biopsies from 20 clinical cases and 7 healthy control dogs were used to extract mRNA. TLR2 and TLR4 mRNA expression was assessed using quantitative real-time PCR. TLR2 mRNA expression was significantly increased in the IBD dogs compared to controls, whereas TLR4 mRNA expression was similar in IBD and control cases. In addition, TLR2 mRNA expression was mildly correlated with clinical severity of disease, however, there was no correlation between TLR2 expression and histological severity of disease. (C) 2009 Elsevier B.V. All rights reserved.

AB - There is growing evidence that aberrant innate immune responses towards the bacterial flora of the gut play a role in the pathogenesis of canine inflammatory bowel disease (IBD). Toll-like receptors (TLR) play an important role as primary sensors of invading pathogens and have gained significant attention in human IBD as differential expression and polymorphisms of certain TLR have been shown to occur in ulcerative colitis (DC) and Crohn's disease (CD).The aim of the current study was to evaluate the expression of two TLR important for recognition of commensals in the gut. TLR2 and TLR4 mRNA expression in duodenal biopsies from dogs with IBD was measured and correlated with clinical and histological disease severity. Endoscopic duodenal biopsies from 20 clinical cases and 7 healthy control dogs were used to extract mRNA. TLR2 and TLR4 mRNA expression was assessed using quantitative real-time PCR. TLR2 mRNA expression was significantly increased in the IBD dogs compared to controls, whereas TLR4 mRNA expression was similar in IBD and control cases. In addition, TLR2 mRNA expression was mildly correlated with clinical severity of disease, however, there was no correlation between TLR2 expression and histological severity of disease. (C) 2009 Elsevier B.V. All rights reserved.

KW - Toll-like receptors

KW - Dog

KW - Inflammatory bowel disease

KW - CHRONIC ENTEROPATHIES

KW - TLR4

KW - COLITIS

KW - HEALTH

KW - CATS

KW - GUT

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JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1-2

ER -

Expression of Toll-like receptor 2 in duodenal biopsies from dogs with inflammatory bowel disease is associated with severity of disease

Abstract

Funding

Keywords

UN SDGs

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