Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry

Sara Rosati; Rebecca J. Rose; Natalie J. Thompson; Esther Van Duijn; Eugen Damoc; Eduard Denisov; Alexander Makarov; Albert J.R. Heck

doi:10.1002/anie.201206745

Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry

Sara Rosati^*
, Rebecca J. Rose
, Natalie J. Thompson
, Esther Van Duijn
, Eugen Damoc
, Eduard Denisov
, Alexander Makarov
, Albert J.R. Heck

^*Corresponding author for this work

Thermo Fisher Scientific

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Monoclonal antibodies (mAbs) represent a major class of therapeutic biomolecules being developed by the biopharmaceutical industry, their popularity largely being a result of their high specificity towards targets. Therapeutic mAbs are being developed for various diseases, with a strong focus on cancer and immunological disorders.[1] All antibody drugs currently approved for clinical use are based on the immunoglobulin G (IgG) class; these molecules are composed of two heavy chains and two light chains, connected by disulfide bonds, and with a molecular weight of approximately 150 kDa. Antibody-based biopharmaceuticals can be complex and heterogeneous molecules, and a range of analytical tools are necessary to characterize them fully.

Original language	English
Pages (from-to)	12992-12996
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	52
DOIs	https://doi.org/10.1002/anie.201206745
Publication status	Published - 21 Dec 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Analytical methods
Antibodies
Biopharmaceuticals
Native mass spectrometry
Orbitrap

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10.1002/anie.201206745

Cite this

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title = "Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry",

abstract = "Monoclonal antibodies (mAbs) represent a major class of therapeutic biomolecules being developed by the biopharmaceutical industry, their popularity largely being a result of their high specificity towards targets. Therapeutic mAbs are being developed for various diseases, with a strong focus on cancer and immunological disorders.[1] All antibody drugs currently approved for clinical use are based on the immunoglobulin G (IgG) class; these molecules are composed of two heavy chains and two light chains, connected by disulfide bonds, and with a molecular weight of approximately 150 kDa. Antibody-based biopharmaceuticals can be complex and heterogeneous molecules, and a range of analytical tools are necessary to characterize them fully.",

keywords = "Analytical methods, Antibodies, Biopharmaceuticals, Native mass spectrometry, Orbitrap",

author = "Sara Rosati and Rose, \{Rebecca J.\} and Thompson, \{Natalie J.\} and \{Van Duijn\}, Esther and Eugen Damoc and Eduard Denisov and Alexander Makarov and Heck, \{Albert J.R.\}",

year = "2012",

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doi = "10.1002/anie.201206745",

language = "English",

volume = "51",

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T1 - Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry

AU - Rosati, Sara

AU - Rose, Rebecca J.

AU - Thompson, Natalie J.

AU - Van Duijn, Esther

AU - Damoc, Eugen

AU - Denisov, Eduard

AU - Makarov, Alexander

AU - Heck, Albert J.R.

PY - 2012/12/21

Y1 - 2012/12/21

N2 - Monoclonal antibodies (mAbs) represent a major class of therapeutic biomolecules being developed by the biopharmaceutical industry, their popularity largely being a result of their high specificity towards targets. Therapeutic mAbs are being developed for various diseases, with a strong focus on cancer and immunological disorders.[1] All antibody drugs currently approved for clinical use are based on the immunoglobulin G (IgG) class; these molecules are composed of two heavy chains and two light chains, connected by disulfide bonds, and with a molecular weight of approximately 150 kDa. Antibody-based biopharmaceuticals can be complex and heterogeneous molecules, and a range of analytical tools are necessary to characterize them fully.

AB - Monoclonal antibodies (mAbs) represent a major class of therapeutic biomolecules being developed by the biopharmaceutical industry, their popularity largely being a result of their high specificity towards targets. Therapeutic mAbs are being developed for various diseases, with a strong focus on cancer and immunological disorders.[1] All antibody drugs currently approved for clinical use are based on the immunoglobulin G (IgG) class; these molecules are composed of two heavy chains and two light chains, connected by disulfide bonds, and with a molecular weight of approximately 150 kDa. Antibody-based biopharmaceuticals can be complex and heterogeneous molecules, and a range of analytical tools are necessary to characterize them fully.

KW - Analytical methods

KW - Antibodies

KW - Biopharmaceuticals

KW - Native mass spectrometry

KW - Orbitrap

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U2 - 10.1002/anie.201206745

DO - 10.1002/anie.201206745

M3 - Article

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AN - SCOPUS:84871270725

SN - 1433-7851

VL - 51

SP - 12992

EP - 12996

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 52

ER -

Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry

Abstract

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Keywords

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