Experiments in membrane protein crystallization

P. Gros; H. Groendijk; J. Drenth; W. G.J. Hol

doi:10.1016/0022-0248(88)90315-6

Experiments in membrane protein crystallization

P. Gros^*, H. Groendijk, J. Drenth, W. G.J. Hol

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Experiments have been carried out aimed at obtaining crystals of three integral membrane proteins: phospholipase A, cytochrome-c reductase and cytochrome-c oxidase. Crystals, in the form of platelets and bipyramids, as well as several micro-crystal forms have been obtained of phospholipase A from the outer membrane of Escherichia coli. The best crystals obtained so far were thin plates having spacegroup C222₁ with a = 72.0 Å, b = 107 Å, c = 75.8 Å and one molecule per asymmetric unit. Reflections were observed out to 2.7 Å resolution. Long red needles have been obtained of the respiratory chain complex-III, or cytochrome-c reductase, from beefheart.

Original language	English
Pages (from-to)	193-200
Number of pages	8
Journal	Journal of Crystal Growth
Volume	90
Issue number	1-3
DOIs	https://doi.org/10.1016/0022-0248(88)90315-6
Publication status	Published - 2 Jul 1988
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by The Netherlands Foundation for Chemical Research (SON) with financial aid from The Netherlands Organization for the Advancement of Pure Research (ZWO).

Funding

This research was supported by The Netherlands Foundation for Chemical Research (SON) with financial aid from The Netherlands Organization for the Advancement of Pure Research (ZWO).

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10.1016/0022-0248(88)90315-6

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title = "Experiments in membrane protein crystallization",

abstract = "Experiments have been carried out aimed at obtaining crystals of three integral membrane proteins: phospholipase A, cytochrome-c reductase and cytochrome-c oxidase. Crystals, in the form of platelets and bipyramids, as well as several micro-crystal forms have been obtained of phospholipase A from the outer membrane of Escherichia coli. The best crystals obtained so far were thin plates having spacegroup C2221 with a = 72.0 {\AA}, b = 107 {\AA}, c = 75.8 {\AA} and one molecule per asymmetric unit. Reflections were observed out to 2.7 {\AA} resolution. Long red needles have been obtained of the respiratory chain complex-III, or cytochrome-c reductase, from beefheart.",

author = "P. Gros and H. Groendijk and J. Drenth and Hol, {W. G.J.}",

note = "Funding Information: This research was supported by The Netherlands Foundation for Chemical Research (SON) with financial aid from The Netherlands Organization for the Advancement of Pure Research (ZWO).",

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doi = "10.1016/0022-0248(88)90315-6",

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T1 - Experiments in membrane protein crystallization

AU - Gros, P.

AU - Groendijk, H.

AU - Drenth, J.

AU - Hol, W. G.J.

N1 - Funding Information: This research was supported by The Netherlands Foundation for Chemical Research (SON) with financial aid from The Netherlands Organization for the Advancement of Pure Research (ZWO).

PY - 1988/7/2

Y1 - 1988/7/2

N2 - Experiments have been carried out aimed at obtaining crystals of three integral membrane proteins: phospholipase A, cytochrome-c reductase and cytochrome-c oxidase. Crystals, in the form of platelets and bipyramids, as well as several micro-crystal forms have been obtained of phospholipase A from the outer membrane of Escherichia coli. The best crystals obtained so far were thin plates having spacegroup C2221 with a = 72.0 Å, b = 107 Å, c = 75.8 Å and one molecule per asymmetric unit. Reflections were observed out to 2.7 Å resolution. Long red needles have been obtained of the respiratory chain complex-III, or cytochrome-c reductase, from beefheart.

AB - Experiments have been carried out aimed at obtaining crystals of three integral membrane proteins: phospholipase A, cytochrome-c reductase and cytochrome-c oxidase. Crystals, in the form of platelets and bipyramids, as well as several micro-crystal forms have been obtained of phospholipase A from the outer membrane of Escherichia coli. The best crystals obtained so far were thin plates having spacegroup C2221 with a = 72.0 Å, b = 107 Å, c = 75.8 Å and one molecule per asymmetric unit. Reflections were observed out to 2.7 Å resolution. Long red needles have been obtained of the respiratory chain complex-III, or cytochrome-c reductase, from beefheart.

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DO - 10.1016/0022-0248(88)90315-6

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Experiments in membrane protein crystallization

Abstract

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