Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell-Cycle Exit after DNA Damage

Hendrika A. Segeren; Lotte M. van Rijnberk; Eva Moreno; Frank M. Riemers; Elsbeth A. van Liere; Ruixue Yuan; Richard Wubbolts; Alain de Bruin; Bart Westendorp

doi:10.1016/j.celrep.2020.108449

Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell-Cycle Exit after DNA Damage

Hendrika A. Segeren, Lotte M. van Rijnberk, Eva Moreno, Frank M. Riemers, Elsbeth A. van Liere, Ruixue Yuan, Richard Wubbolts, Alain de Bruin, Bart Westendorp

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Graphical Abstract Highlights d Individual cycling cancer cells display enhanced E2F target gene expression d E2F7/8 deletion or E2F3 overexpression overrides cell-cycle exit after DNA damage d Elevated levels of the E2F target Emi1 prevent DNA-damage-induced cell-cycle exit d The cell-cycle exit after DNA damage is transient and leads to endoreplication

Original language	English
Article number	108449
Number of pages	21
Journal	Cell Reports
Volume	33
Issue number	9
DOIs	https://doi.org/10.1016/j.celrep.2020.108449
Publication status	Published - 1 Dec 2020

Keywords

cell cycle
anaphase-promoting complex/cyclosome
E2F transcription
live-cell imaging
single-cell RNA sequencing
DNA damage
cancer
P53
Emi1
tetraploidy

Access to Document

10.1016/j.celrep.2020.108449

1-s2.0-S2211124720314388-main (1)Final published version, 6.97 MBLicence: CC BY-NC-ND

Cite this

@article{826a85b75f8d4776b19d80f9344fc263,

title = "Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell-Cycle Exit after DNA Damage",

abstract = "Graphical Abstract Highlights d Individual cycling cancer cells display enhanced E2F target gene expression d E2F7/8 deletion or E2F3 overexpression overrides cell-cycle exit after DNA damage d Elevated levels of the E2F target Emi1 prevent DNA-damage-induced cell-cycle exit d The cell-cycle exit after DNA damage is transient and leads to endoreplication",

keywords = "cell cycle, anaphase-promoting complex/cyclosome, E2F transcription, live-cell imaging, single-cell RNA sequencing, DNA damage, cancer, P53, Emi1, tetraploidy",

author = "Segeren, {Hendrika A.} and {van Rijnberk}, {Lotte M.} and Eva Moreno and Riemers, {Frank M.} and {van Liere}, {Elsbeth A.} and Ruixue Yuan and Richard Wubbolts and {de Bruin}, Alain and Bart Westendorp",

year = "2020",

month = dec,

day = "1",

doi = "10.1016/j.celrep.2020.108449",

language = "English",

volume = "33",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Elsevier Saunders",

number = "9",

}

TY - JOUR

T1 - Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell-Cycle Exit after DNA Damage

AU - Segeren, Hendrika A.

AU - van Rijnberk, Lotte M.

AU - Moreno, Eva

AU - Riemers, Frank M.

AU - van Liere, Elsbeth A.

AU - Yuan, Ruixue

AU - Wubbolts, Richard

AU - de Bruin, Alain

AU - Westendorp, Bart

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Graphical Abstract Highlights d Individual cycling cancer cells display enhanced E2F target gene expression d E2F7/8 deletion or E2F3 overexpression overrides cell-cycle exit after DNA damage d Elevated levels of the E2F target Emi1 prevent DNA-damage-induced cell-cycle exit d The cell-cycle exit after DNA damage is transient and leads to endoreplication

AB - Graphical Abstract Highlights d Individual cycling cancer cells display enhanced E2F target gene expression d E2F7/8 deletion or E2F3 overexpression overrides cell-cycle exit after DNA damage d Elevated levels of the E2F target Emi1 prevent DNA-damage-induced cell-cycle exit d The cell-cycle exit after DNA damage is transient and leads to endoreplication

KW - cell cycle

KW - anaphase-promoting complex/cyclosome

KW - E2F transcription

KW - live-cell imaging

KW - single-cell RNA sequencing

KW - DNA damage

KW - cancer

KW - P53

KW - Emi1

KW - tetraploidy

UR - https://www.mendeley.com/catalogue/f3cddff1-d5f7-36e4-afa1-673149508e79/

U2 - 10.1016/j.celrep.2020.108449

DO - 10.1016/j.celrep.2020.108449

M3 - Article

C2 - 33264622

SN - 2211-1247

VL - 33

JO - Cell Reports

JF - Cell Reports

IS - 9

M1 - 108449

ER -

Excessive E2F Transcription in Single Cancer Cells Precludes Transient Cell-Cycle Exit after DNA Damage

Abstract

Keywords

Access to Document

Fingerprint

Cite this