Evaluation of suspension array analysis for detection of egg yolk antibodies against Salmonella Enteritidis.

M.E. Thomas, D. Klinkenberg, A. Bergwerff, E. van Eerden, J.A. Stegeman, A. Bouma

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Salmonella enterica serovar Enteritidis (SE) is an important source of food-related diarrhoea in humans, and table eggs are considered the primordial source of contamination of the human food chain. Using eggs collected at egg-packing stations as samples could be a convenient strategy to detect colonization of layer flocks. The aim of this study was to evaluate egg yolk anti-Salmonella antibody detection using suspension array analysis. An egg yolk panel from contact-infected and non-colonized laying hens was used for the evaluation. Receiver Operating Characteristic (ROC) curves were generated to define a cut-off value and to assess the overall accuracy of the assay. The diagnostic sensitivity and specificity were estimated by maximum likelihood. Sensitivity was quantified on hen level and on sample level, and also quantified as a function of time since colonization. The area under the ROC curve was estimated at 0.984 (se 0.006, P
    Original languageEnglish
    Pages (from-to)137-143
    Number of pages7
    JournalPreventive Veterinary Medicine
    Volume95
    Issue number1-2
    DOIs
    Publication statusPublished - 2010

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