Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context

E. Dinant Kroese; Sieto Bosgra; Harrie E. Buist; Geertje Lewin; Sander C. van der Linden; Hai-yen Man; Aldert H. Piersma; Emiel Rorije; Sjors H. W. Schulpen; Michael Schwarz; Frederik Uibel; Barbara M. A. van Vugt-Lussenburg; Andre P. M. Wolterbeek; Bart van der Burg

doi:10.1016/j.reprotox.2014.10.003

Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context

E. Dinant Kroese^*, Sieto Bosgra, Harrie E. Buist, Geertje Lewin, Sander C. van der Linden, Hai-yen Man, Aldert H. Piersma, Emiel Rorije, Sjors H. W. Schulpen, Michael Schwarz, Frederik Uibel, Barbara M. A. van Vugt-Lussenburg, Andre P. M. Wolterbeek, Bart van der Burg

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Previously we showed a battery consisting of CALUX transcriptional activation assays, the ReProGlo assay, and the embryonic stem cell test, and zebrafish embryotoxicity assay as 'apical' tests to correctly predict developmental toxicity for 11 out of 12 compounds, and to explain the one false negative [7]. Here we report on applying this battery within the context of grouping and read across, put forward as a potential tool to fill data gaps and avoid animal testing, to distinguish in vivo non-or weak developmental toxicants from potent developmental toxicants within groups of structural analogs. The battery correctly distinguished 2-methylhexanoic acid, monomethyl phthalate, and monobutyltin trichloride as non-or weak developmental toxicants from structurally related developmental toxicants valproic acid, mono-ethylhexyl phthalate, and tributyltin chloride, respectively, and, therefore, holds promise as a biological verification model in grouping and read across approaches. The relevance of toxicokinetic information is indicated. (C) 2014 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	11-19
Number of pages	9
Journal	Reproductive Toxicology
Volume	55
DOIs	https://doi.org/10.1016/j.reprotox.2014.10.003
Publication status	Published - 1 Aug 2015

Funding

This work was partially financed by the Commission of the European Communities, the collaborative project ChemScreen (GA244236).

Keywords

Alternative model
Developmental toxicity
Read across
Grouping
CALUX
Embryonic stem cell test
Zebrafish embryotoxicity assay
ReProGlo
BUTYL BENZYL PHTHALATE
VALPROIC ACID VPA
DEVELOPMENTAL TOXICITY
AROMATASE-ACTIVITY
CELL-LINE
RATS
EMBRYOTOXICITY
CHEMICALS
DIFFERENTIATION
TERATOGENICITY

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10.1016/j.reprotox.2014.10.003

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Kroese, E. D., Bosgra, S., Buist, H. E., Lewin, G., van der Linden, S. C., Man, H., Piersma, A. H., Rorije, E., Schulpen, S. H. W., Schwarz, M., Uibel, F., van Vugt-Lussenburg, B. M. A., Wolterbeek, A. P. M., & van der Burg, B. (2015). Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context. Reproductive Toxicology, 55, 11-19. https://doi.org/10.1016/j.reprotox.2014.10.003

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title = "Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context",

abstract = "Previously we showed a battery consisting of CALUX transcriptional activation assays, the ReProGlo assay, and the embryonic stem cell test, and zebrafish embryotoxicity assay as 'apical' tests to correctly predict developmental toxicity for 11 out of 12 compounds, and to explain the one false negative [7]. Here we report on applying this battery within the context of grouping and read across, put forward as a potential tool to fill data gaps and avoid animal testing, to distinguish in vivo non-or weak developmental toxicants from potent developmental toxicants within groups of structural analogs. The battery correctly distinguished 2-methylhexanoic acid, monomethyl phthalate, and monobutyltin trichloride as non-or weak developmental toxicants from structurally related developmental toxicants valproic acid, mono-ethylhexyl phthalate, and tributyltin chloride, respectively, and, therefore, holds promise as a biological verification model in grouping and read across approaches. The relevance of toxicokinetic information is indicated. (C) 2014 Elsevier Inc. All rights reserved.",

keywords = "Alternative model, Developmental toxicity, Read across, Grouping, CALUX, Embryonic stem cell test, Zebrafish embryotoxicity assay, ReProGlo, BUTYL BENZYL PHTHALATE, VALPROIC ACID VPA, DEVELOPMENTAL TOXICITY, AROMATASE-ACTIVITY, CELL-LINE, RATS, EMBRYOTOXICITY, CHEMICALS, DIFFERENTIATION, TERATOGENICITY",

author = "Kroese, {E. Dinant} and Sieto Bosgra and Buist, {Harrie E.} and Geertje Lewin and {van der Linden}, {Sander C.} and Hai-yen Man and Piersma, {Aldert H.} and Emiel Rorije and Schulpen, {Sjors H. W.} and Michael Schwarz and Frederik Uibel and {van Vugt-Lussenburg}, {Barbara M. A.} and Wolterbeek, {Andre P. M.} and {van der Burg}, Bart",

year = "2015",

month = aug,

day = "1",

doi = "10.1016/j.reprotox.2014.10.003",

language = "English",

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journal = "Reproductive Toxicology",

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Kroese, ED, Bosgra, S, Buist, HE, Lewin, G, van der Linden, SC, Man, H, Piersma, AH, Rorije, E, Schulpen, SHW, Schwarz, M, Uibel, F, van Vugt-Lussenburg, BMA, Wolterbeek, APM & van der Burg, B 2015, 'Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context', Reproductive Toxicology, vol. 55, pp. 11-19. https://doi.org/10.1016/j.reprotox.2014.10.003

TY - JOUR

T1 - Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context

AU - Kroese, E. Dinant

AU - Bosgra, Sieto

AU - Buist, Harrie E.

AU - Lewin, Geertje

AU - van der Linden, Sander C.

AU - Man, Hai-yen

AU - Piersma, Aldert H.

AU - Rorije, Emiel

AU - Schulpen, Sjors H. W.

AU - Schwarz, Michael

AU - Uibel, Frederik

AU - van Vugt-Lussenburg, Barbara M. A.

AU - Wolterbeek, Andre P. M.

AU - van der Burg, Bart

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Previously we showed a battery consisting of CALUX transcriptional activation assays, the ReProGlo assay, and the embryonic stem cell test, and zebrafish embryotoxicity assay as 'apical' tests to correctly predict developmental toxicity for 11 out of 12 compounds, and to explain the one false negative [7]. Here we report on applying this battery within the context of grouping and read across, put forward as a potential tool to fill data gaps and avoid animal testing, to distinguish in vivo non-or weak developmental toxicants from potent developmental toxicants within groups of structural analogs. The battery correctly distinguished 2-methylhexanoic acid, monomethyl phthalate, and monobutyltin trichloride as non-or weak developmental toxicants from structurally related developmental toxicants valproic acid, mono-ethylhexyl phthalate, and tributyltin chloride, respectively, and, therefore, holds promise as a biological verification model in grouping and read across approaches. The relevance of toxicokinetic information is indicated. (C) 2014 Elsevier Inc. All rights reserved.

AB - Previously we showed a battery consisting of CALUX transcriptional activation assays, the ReProGlo assay, and the embryonic stem cell test, and zebrafish embryotoxicity assay as 'apical' tests to correctly predict developmental toxicity for 11 out of 12 compounds, and to explain the one false negative [7]. Here we report on applying this battery within the context of grouping and read across, put forward as a potential tool to fill data gaps and avoid animal testing, to distinguish in vivo non-or weak developmental toxicants from potent developmental toxicants within groups of structural analogs. The battery correctly distinguished 2-methylhexanoic acid, monomethyl phthalate, and monobutyltin trichloride as non-or weak developmental toxicants from structurally related developmental toxicants valproic acid, mono-ethylhexyl phthalate, and tributyltin chloride, respectively, and, therefore, holds promise as a biological verification model in grouping and read across approaches. The relevance of toxicokinetic information is indicated. (C) 2014 Elsevier Inc. All rights reserved.

KW - Alternative model

KW - Developmental toxicity

KW - Read across

KW - Grouping

KW - CALUX

KW - Embryonic stem cell test

KW - Zebrafish embryotoxicity assay

KW - ReProGlo

KW - BUTYL BENZYL PHTHALATE

KW - VALPROIC ACID VPA

KW - DEVELOPMENTAL TOXICITY

KW - AROMATASE-ACTIVITY

KW - CELL-LINE

KW - RATS

KW - EMBRYOTOXICITY

KW - CHEMICALS

KW - DIFFERENTIATION

KW - TERATOGENICITY

U2 - 10.1016/j.reprotox.2014.10.003

DO - 10.1016/j.reprotox.2014.10.003

M3 - Article

SN - 0890-6238

VL - 55

SP - 11

EP - 19

JO - Reproductive Toxicology

JF - Reproductive Toxicology

ER -

Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context

Abstract

Funding

Keywords

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