Eucalypt species drive rhizosphere bacterial and fungal community assembly but soil phosphorus availability rearranges the microbiome

R. G. Bulgarelli; M. F.A. Leite; M. de Hollander; P. Mazzafera; S. A.L. Andrade; E. E. Kuramae

doi:10.1016/j.scitotenv.2022.155667

Eucalypt species drive rhizosphere bacterial and fungal community assembly but soil phosphorus availability rearranges the microbiome

R. G. Bulgarelli, M. F.A. Leite, M. de Hollander, P. Mazzafera, S. A.L. Andrade^*, E. E. Kuramae

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Soil phosphorus (P) availability may limit plant growth and alter root-soil interactions and rhizosphere microbial community composition. The composition of the rhizosphere microbial community can also be shaped by plant genotype. In this study, we examined the rhizosphere microbial communities of young plants of 24 species of eucalypts (22 Eucalyptus and two Corymbia species) under low or sufficient soil P availability. The taxonomic diversity of the rhizosphere bacterial and fungal communities was assessed by 16S and 18S rRNA gene amplicon sequencing. The taxonomic modifications in response to low P availability were evaluated by principal component analysis, and co-inertia analysis was performed to identify associations between bacterial and fungal community structures and parameters related to plant growth and nutritional status under low and sufficient soil P availability. The sequencing results showed that while both soil P availability and eucalypt species influenced the microbial community assembly, eucalypt species was the stronger determinant. However, when the plants are subjected to low P-availability, the rhizosphere selection became strongest. In response to low P, the bacterial and fungal communities in the rhizosphere of some species showed significant changes, whereas in others remained relatively constant under low and sufficient P. Co-inertia analyses revealed a significant co-dependence between plant nutrient contents and bacterial and fungal community composition only under sufficient P. By contrast, under low P, bacterial community composition was related to plant biomass production. In conclusion, our study shows that eucalypt species identity was the main factor modulating rhizosphere microbial community composition; significant shifts due to P availability were observed only for some eucalypt species.

Original language	English
Article number	155667
Number of pages	10
Journal	Science of the Total Environment
Volume	836
DOIs	https://doi.org/10.1016/j.scitotenv.2022.155667
Publication status	Published - 25 Aug 2022

Bibliographical note

Funding Information:
This work was supported by the São Paulo Research Foundation (FAPESP – Grant number 2016/25498-0 ). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW).

Funding Information:
We thank FAPESP and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil) for doctoral and doctorate-sandwich program abroad fellowships to RGB. PM thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for a research fellowship.

Funding Information:
This work was supported by the São Paulo Research Foundation (FAPESP – Grant number 2016/25498-0). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW).

Publisher Copyright:
© 2022 Elsevier B.V.

Funding

This work was supported by the São Paulo Research Foundation (FAPESP – Grant number 2016/25498-0 ). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW). We thank FAPESP and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil) for doctoral and doctorate-sandwich program abroad fellowships to RGB. PM thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for a research fellowship. This work was supported by the São Paulo Research Foundation (FAPESP – Grant number 2016/25498-0). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW).

Keywords

16S rRNA gene
18S rRNA gene
Next-generation sequencing
Plant species

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10.1016/j.scitotenv.2022.155667

1-s2.0-S0048969722027632-mainFinal published version, 2.01 MBLicence: Taverne

Cite this

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title = "Eucalypt species drive rhizosphere bacterial and fungal community assembly but soil phosphorus availability rearranges the microbiome",

abstract = "Soil phosphorus (P) availability may limit plant growth and alter root-soil interactions and rhizosphere microbial community composition. The composition of the rhizosphere microbial community can also be shaped by plant genotype. In this study, we examined the rhizosphere microbial communities of young plants of 24 species of eucalypts (22 Eucalyptus and two Corymbia species) under low or sufficient soil P availability. The taxonomic diversity of the rhizosphere bacterial and fungal communities was assessed by 16S and 18S rRNA gene amplicon sequencing. The taxonomic modifications in response to low P availability were evaluated by principal component analysis, and co-inertia analysis was performed to identify associations between bacterial and fungal community structures and parameters related to plant growth and nutritional status under low and sufficient soil P availability. The sequencing results showed that while both soil P availability and eucalypt species influenced the microbial community assembly, eucalypt species was the stronger determinant. However, when the plants are subjected to low P-availability, the rhizosphere selection became strongest. In response to low P, the bacterial and fungal communities in the rhizosphere of some species showed significant changes, whereas in others remained relatively constant under low and sufficient P. Co-inertia analyses revealed a significant co-dependence between plant nutrient contents and bacterial and fungal community composition only under sufficient P. By contrast, under low P, bacterial community composition was related to plant biomass production. In conclusion, our study shows that eucalypt species identity was the main factor modulating rhizosphere microbial community composition; significant shifts due to P availability were observed only for some eucalypt species.",

keywords = "16S rRNA gene, 18S rRNA gene, Next-generation sequencing, Plant species",

author = "Bulgarelli, {R. G.} and Leite, {M. F.A.} and {de Hollander}, M. and P. Mazzafera and Andrade, {S. A.L.} and Kuramae, {E. E.}",

note = "Funding Information: This work was supported by the S{\~a}o Paulo Research Foundation (FAPESP – Grant number 2016/25498-0 ). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW). Funding Information: We thank FAPESP and CAPES (Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior - Brazil) for doctoral and doctorate-sandwich program abroad fellowships to RGB. PM thanks Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) for a research fellowship. Funding Information: This work was supported by the S{\~a}o Paulo Research Foundation (FAPESP – Grant number 2016/25498-0). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

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AU - Leite, M. F.A.

AU - de Hollander, M.

AU - Mazzafera, P.

AU - Andrade, S. A.L.

AU - Kuramae, E. E.

N1 - Funding Information: This work was supported by the São Paulo Research Foundation (FAPESP – Grant number 2016/25498-0 ). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW). Funding Information: We thank FAPESP and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil) for doctoral and doctorate-sandwich program abroad fellowships to RGB. PM thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for a research fellowship. Funding Information: This work was supported by the São Paulo Research Foundation (FAPESP – Grant number 2016/25498-0). Publication number 7420 of the Netherlands Institute of Ecology (NIOO-KNAW). Publisher Copyright: © 2022 Elsevier B.V.

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N2 - Soil phosphorus (P) availability may limit plant growth and alter root-soil interactions and rhizosphere microbial community composition. The composition of the rhizosphere microbial community can also be shaped by plant genotype. In this study, we examined the rhizosphere microbial communities of young plants of 24 species of eucalypts (22 Eucalyptus and two Corymbia species) under low or sufficient soil P availability. The taxonomic diversity of the rhizosphere bacterial and fungal communities was assessed by 16S and 18S rRNA gene amplicon sequencing. The taxonomic modifications in response to low P availability were evaluated by principal component analysis, and co-inertia analysis was performed to identify associations between bacterial and fungal community structures and parameters related to plant growth and nutritional status under low and sufficient soil P availability. The sequencing results showed that while both soil P availability and eucalypt species influenced the microbial community assembly, eucalypt species was the stronger determinant. However, when the plants are subjected to low P-availability, the rhizosphere selection became strongest. In response to low P, the bacterial and fungal communities in the rhizosphere of some species showed significant changes, whereas in others remained relatively constant under low and sufficient P. Co-inertia analyses revealed a significant co-dependence between plant nutrient contents and bacterial and fungal community composition only under sufficient P. By contrast, under low P, bacterial community composition was related to plant biomass production. In conclusion, our study shows that eucalypt species identity was the main factor modulating rhizosphere microbial community composition; significant shifts due to P availability were observed only for some eucalypt species.

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KW - 18S rRNA gene

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Eucalypt species drive rhizosphere bacterial and fungal community assembly but soil phosphorus availability rearranges the microbiome

Abstract

Bibliographical note

Funding

Keywords

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