Epigenetic regulation of repetitive elements is attenuated by prolonged heat stress in Arabidopsis

Ales Pecinka, Huy Q Dinh, Tuncay Baubec, Marisa Rosa, Nicole Lettner, Ortrun Mittelsten Scheid

Research output: Contribution to journalArticleAcademicpeer-review


Epigenetic factors determine responses to internal and external stimuli in eukaryotic organisms. Whether and how environmental conditions feed back to the epigenetic landscape is more a matter of suggestion than of substantiation. Plants are suitable organisms with which to address this question due to their sessile lifestyle and diversification of epigenetic regulators. We show that several repetitive elements of Arabidopsis thaliana that are under epigenetic regulation by transcriptional gene silencing at ambient temperatures and upon short term heat exposure become activated by prolonged heat stress. Activation can occur without loss of DNA methylation and with only minor changes to histone modifications but is accompanied by loss of nucleosomes and by heterochromatin decondensation. Whereas decondensation persists, nucleosome loading and transcriptional silencing are restored upon recovery from heat stress but are delayed in mutants with impaired chromatin assembly functions. The results provide evidence that environmental conditions can override epigenetic regulation, at least transiently, which might open a window for more permanent epigenetic changes.

Original languageEnglish
Pages (from-to)3118-29
Number of pages12
JournalThe Plant Cell
Issue number9
Publication statusPublished - Sept 2010
Externally publishedYes


  • Arabidopsis/genetics
  • DNA Methylation
  • DNA, Plant/metabolism
  • Epigenesis, Genetic
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Gene Silencing
  • Heat-Shock Response
  • Heterochromatin/metabolism
  • Histones/metabolism
  • Hot Temperature
  • Nucleosomes/metabolism
  • Repetitive Sequences, Nucleic Acid
  • Transcription, Genetic
  • Transcriptional Activation


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