Abstract
The cortical polarity regulators PAR-6, PKC-3, and PAR-3 are essential for the polarization of a broad variety of cell types in multicellular animals. In C. elegans, the roles of the PAR proteins in embryonic development have been extensively studied, yet little is known about their functions during larval development. Using inducible protein degradation, we show that PAR-6 and PKC-3, but not PAR-3, are essential for postembryonic development. PAR-6 and PKC-3 are required in the epidermal epithelium for animal growth, molting, and the proper pattern of seam-cell divisions. Finally, we uncovered a novel role for PAR-6 in organizing non-centrosomal microtubule arrays in the epidermis. PAR-6 was required for the localization of the microtubule organizer NOCA-1/Ninein, and defects in a noca-1 mutant are highly similar to those caused by epidermal PAR-6 depletion. As NOCA-1 physically interacts with PAR-6, we propose that PAR-6 promotes non-centrosomal microtubule organization through localization of NOCA-1/Ninein.
| Original language | English |
|---|---|
| Article number | e62067 |
| Pages (from-to) | 1-37 |
| Number of pages | 37 |
| Journal | eLife |
| Volume | 9 |
| DOIs | |
| Publication status | Published - Dec 2020 |
Bibliographical note
Funding Information:We thank R Schmidt and S van den Heuvel for sharing strain SV1937, S van den Heuvel for strain SV1009, J Feldman for strains JLF15 and JLF173, K Oegema for strain OD1652, A Woollard for strain AW1015, A Frand for strain GR1395, and D Fay for strain RT3638. We thank S van den Heuvel, M Harterink, D Fay and members of the S van den Heuvel and M Boxem groups for helpful discussions, M Harterink for critical reading of the manuscript, J Sepers for help generating PAR-3 strains, and J Cravo for generating the rose plots. We also thank Wormbase (Harris et al., 2020) and the Biology Imaging Center, Faculty of Sciences, Department of Biology, Utrecht University. Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). This work was supported by the Netherlands Organization for Scientific Research (NWO)-ALW Open Program 824.14.021 and NWO-VICI 016.VICI.170.165 grants to M Boxem, and the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 675407 – PolarNet.
Publisher Copyright:
© Castiglioni et al.
Funding
We thank R Schmidt and S van den Heuvel for sharing strain SV1937, S van den Heuvel for strain SV1009, J Feldman for strains JLF15 and JLF173, K Oegema for strain OD1652, A Woollard for strain AW1015, A Frand for strain GR1395, and D Fay for strain RT3638. We thank S van den Heuvel, M Harterink, D Fay and members of the S van den Heuvel and M Boxem groups for helpful discussions, M Harterink for critical reading of the manuscript, J Sepers for help generating PAR-3 strains, and J Cravo for generating the rose plots. We also thank Wormbase (Harris et al., 2020) and the Biology Imaging Center, Faculty of Sciences, Department of Biology, Utrecht University. Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). This work was supported by the Netherlands Organization for Scientific Research (NWO)-ALW Open Program 824.14.021 and NWO-VICI 016.VICI.170.165 grants to M Boxem, and the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 675407 – PolarNet.
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