TY - JOUR
T1 - Enzymatic Decoration of Prebiotic Galacto-oligosaccharides (Vivinal GOS) with Sialic Acid Using Trypanosoma cruzi trans -Sialidase and Two Bovine Sialoglycoconjugates as Donor Substrates
AU - Wilbrink, Maarten H.
AU - Ten Kate, Geert A.
AU - Sanders, Peter
AU - Gerwig, Gerrit J.
AU - Van Leeuwen, Sander S.
AU - Sallomons, Erik
AU - Klarenbeek, Bert
AU - Hage, Johannes A.
AU - Van Vuure, Carine A.
AU - Dijkhuizen, Lubbert
AU - Kamerling, Johannis P.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Decoration of prebiotic galacto-oligosaccharides (GOS) with sialic acid yields mixtures of GOS and sialylated GOS (Sia-GOS), novel products that are expected to have both prebiotic and antiadhesive functionalities. The recombinantly produced trans-sialidase enzyme from Trypanosoma cruzi (TcTS), an enzyme with the ability to transfer (α2-3)-linked sialic acid from sialogalactoglycans to asialogalactoglycans, was employed to catalyze this sialylation. As sialic acid acceptor substrates, Vivinal GOS and derived fractions of specific degree of polymerization were taken. As sialic acid donor substrates, bovine κ-casein-derived glycomacropeptide [>99% N-acetylneuraminic acid (Neu5Ac); <1% N-glycolylneuraminic acid (Neu5Gc)] and bovine blood plasma glycoprotein mixture (45% Neu5Ac; 55% Neu5Gc) were selected, yielding potential food and feed products, respectively. High-pH anion-exchange chromatography, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy were used for product analysis.
AB - Decoration of prebiotic galacto-oligosaccharides (GOS) with sialic acid yields mixtures of GOS and sialylated GOS (Sia-GOS), novel products that are expected to have both prebiotic and antiadhesive functionalities. The recombinantly produced trans-sialidase enzyme from Trypanosoma cruzi (TcTS), an enzyme with the ability to transfer (α2-3)-linked sialic acid from sialogalactoglycans to asialogalactoglycans, was employed to catalyze this sialylation. As sialic acid acceptor substrates, Vivinal GOS and derived fractions of specific degree of polymerization were taken. As sialic acid donor substrates, bovine κ-casein-derived glycomacropeptide [>99% N-acetylneuraminic acid (Neu5Ac); <1% N-glycolylneuraminic acid (Neu5Gc)] and bovine blood plasma glycoprotein mixture (45% Neu5Ac; 55% Neu5Gc) were selected, yielding potential food and feed products, respectively. High-pH anion-exchange chromatography, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy were used for product analysis.
KW - bovine blood plasma glycoprotein
KW - bovine glycomacropeptide
KW - galacto-oligosaccharides
KW - sialic acid
KW - trans-sialidase
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=84935075865&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b01505
DO - 10.1021/acs.jafc.5b01505
M3 - Article
C2 - 26044147
AN - SCOPUS:84935075865
SN - 0021-8561
VL - 63
SP - 5976
EP - 5984
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 25
ER -