Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation

L. Bastos Sales; J.H. Kamstra; P.H. Cenijn; L.S. van Rijt; T. Hamers; J. Legler

doi:10.1016/j.tiv.2013.04.005

Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation

L. Bastos Sales, J.H. Kamstra, P.H. Cenijn, L.S. van Rijt, T. Hamers, J. Legler

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Recent studies suggest that endocrine disrupting chemicals (EDCs) may form a risk factor for obesity by altering energy metabolism through epigenetic gene regulation. The goal of this study is to investigate the effects of a range of EDCs with putative obesogenic properties on global DNA methylation and adipocyte differentiation in vitro. Murine N2A and human SK-N-AS neuroblastoma cells and murine preadipocyte fibroblasts (3T3-L1) were exposed to tributyltin (TBT), diethylstilbestrol (DES), bisphenol A (BPA), 2,3,7,8-tetrachlorodibenzo-[. p]-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153), hexachlorobenzene (HCB), hexabromocyclododecane (HBCD), 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) , perfluorinated octyl acid (PFOA) and perfluorinated octyl sulfonate (PFOS). A modest decrease in global DNA methylation was observed in N2A cells exposed to 10. μM DES, BPA, TCDD, BDE-47, PCB-153 and 1. μM HCB, but no changes were found in the human SK-N-AS cells. We reveal for the first time that BDE-47 increases adipocyte differentiation in a dose-dependent manner (2.5-25. μM). Adipocyte differentiation was also enhanced by TBT (≥10. nM) and BPA (

Original language	English
Pages (from-to)	1634-1643
Number of pages	10
Journal	Toxicology in Vitro
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.tiv.2013.04.005
Publication status	Published - 1 Sept 2013

Keywords

Adipocyte differentiation
Bromodiphenylether
Endocrine disrupting chemicals
Epigenetics
In vitro
Obesity
Tributyltin
2,2',4',4' tetrabrominated diphenyl ether
2,2',4,4',5,5' hexachlorobiphenyl
2,3,7,8 tetrachlorodibenzo para dioxin
4,4' isopropylidenediphenol
diethylstilbestrol
endocrine disruptor
hexabromocyclododecane
hexachlorobenzene
organobromine derivative
perfluorinated octyl acid
perfluorinated octyl sulfonate
perfluoro compound
tributyltin
unclassified drug
adipocyte
animal cell
article
cancer cell culture
cell differentiation
concentration (parameter)
controlled study
cytotoxicity test
DNA methylation
DNA purification
fibroblast
flow cytometry
gene control
human
human cell
in vitro study
mouse
neuroblastoma cell
nonhuman
polymerase chain reaction
quantitative analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tiv.2013.04.005

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title = "Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation",

abstract = "Recent studies suggest that endocrine disrupting chemicals (EDCs) may form a risk factor for obesity by altering energy metabolism through epigenetic gene regulation. The goal of this study is to investigate the effects of a range of EDCs with putative obesogenic properties on global DNA methylation and adipocyte differentiation in vitro. Murine N2A and human SK-N-AS neuroblastoma cells and murine preadipocyte fibroblasts (3T3-L1) were exposed to tributyltin (TBT), diethylstilbestrol (DES), bisphenol A (BPA), 2,3,7,8-tetrachlorodibenzo-[. p]-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153), hexachlorobenzene (HCB), hexabromocyclododecane (HBCD), 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) , perfluorinated octyl acid (PFOA) and perfluorinated octyl sulfonate (PFOS). A modest decrease in global DNA methylation was observed in N2A cells exposed to 10. μM DES, BPA, TCDD, BDE-47, PCB-153 and 1. μM HCB, but no changes were found in the human SK-N-AS cells. We reveal for the first time that BDE-47 increases adipocyte differentiation in a dose-dependent manner (2.5-25. μM). Adipocyte differentiation was also enhanced by TBT (≥10. nM) and BPA (",

keywords = "Adipocyte differentiation, Bromodiphenylether, Endocrine disrupting chemicals, Epigenetics, In vitro, Obesity, Tributyltin, 2,2',4',4' tetrabrominated diphenyl ether, 2,2',4,4',5,5' hexachlorobiphenyl, 2,3,7,8 tetrachlorodibenzo para dioxin, 4,4' isopropylidenediphenol, diethylstilbestrol, endocrine disruptor, hexabromocyclododecane, hexachlorobenzene, organobromine derivative, perfluorinated octyl acid, perfluorinated octyl sulfonate, perfluoro compound, tributyltin, unclassified drug, adipocyte, animal cell, article, cancer cell culture, cell differentiation, concentration (parameter), controlled study, cytotoxicity test, DNA methylation, DNA purification, fibroblast, flow cytometry, gene control, human, human cell, in vitro study, mouse, neuroblastoma cell, nonhuman, polymerase chain reaction, quantitative analysis",

author = "{Bastos Sales}, L. and J.H. Kamstra and P.H. Cenijn and {van Rijt}, L.S. and T. Hamers and J. Legler",

year = "2013",

month = sep,

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doi = "10.1016/j.tiv.2013.04.005",

language = "English",

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T1 - Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation

AU - Bastos Sales, L.

AU - Kamstra, J.H.

AU - Cenijn, P.H.

AU - van Rijt, L.S.

AU - Hamers, T.

AU - Legler, J.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Recent studies suggest that endocrine disrupting chemicals (EDCs) may form a risk factor for obesity by altering energy metabolism through epigenetic gene regulation. The goal of this study is to investigate the effects of a range of EDCs with putative obesogenic properties on global DNA methylation and adipocyte differentiation in vitro. Murine N2A and human SK-N-AS neuroblastoma cells and murine preadipocyte fibroblasts (3T3-L1) were exposed to tributyltin (TBT), diethylstilbestrol (DES), bisphenol A (BPA), 2,3,7,8-tetrachlorodibenzo-[. p]-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153), hexachlorobenzene (HCB), hexabromocyclododecane (HBCD), 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) , perfluorinated octyl acid (PFOA) and perfluorinated octyl sulfonate (PFOS). A modest decrease in global DNA methylation was observed in N2A cells exposed to 10. μM DES, BPA, TCDD, BDE-47, PCB-153 and 1. μM HCB, but no changes were found in the human SK-N-AS cells. We reveal for the first time that BDE-47 increases adipocyte differentiation in a dose-dependent manner (2.5-25. μM). Adipocyte differentiation was also enhanced by TBT (≥10. nM) and BPA (

AB - Recent studies suggest that endocrine disrupting chemicals (EDCs) may form a risk factor for obesity by altering energy metabolism through epigenetic gene regulation. The goal of this study is to investigate the effects of a range of EDCs with putative obesogenic properties on global DNA methylation and adipocyte differentiation in vitro. Murine N2A and human SK-N-AS neuroblastoma cells and murine preadipocyte fibroblasts (3T3-L1) were exposed to tributyltin (TBT), diethylstilbestrol (DES), bisphenol A (BPA), 2,3,7,8-tetrachlorodibenzo-[. p]-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153), hexachlorobenzene (HCB), hexabromocyclododecane (HBCD), 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) , perfluorinated octyl acid (PFOA) and perfluorinated octyl sulfonate (PFOS). A modest decrease in global DNA methylation was observed in N2A cells exposed to 10. μM DES, BPA, TCDD, BDE-47, PCB-153 and 1. μM HCB, but no changes were found in the human SK-N-AS cells. We reveal for the first time that BDE-47 increases adipocyte differentiation in a dose-dependent manner (2.5-25. μM). Adipocyte differentiation was also enhanced by TBT (≥10. nM) and BPA (

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KW - Obesity

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KW - cancer cell culture

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KW - controlled study

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KW - DNA methylation

KW - DNA purification

KW - fibroblast

KW - flow cytometry

KW - gene control

KW - human

KW - human cell

KW - in vitro study

KW - mouse

KW - neuroblastoma cell

KW - nonhuman

KW - polymerase chain reaction

KW - quantitative analysis

U2 - 10.1016/j.tiv.2013.04.005

DO - 10.1016/j.tiv.2013.04.005

M3 - Article

SN - 0887-2333

VL - 27

SP - 1634

EP - 1643

JO - Toxicology in Vitro

JF - Toxicology in Vitro

IS - 6

ER -

Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation

Abstract

Keywords

UN SDGs

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