Effects of beta-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis

S. G. A. van der Drift; R. R. Everts; M. Houweling; L. A. M. G. van Leengoed; J. A. Stegeman; A. G. M. Tielens; R. Jorritsma

doi:10.1016/j.rvsc.2012.11.009

Effects of beta-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis

S. G. A. van der Drift, R. R. Everts, M. Houweling, L. A. M. G. van Leengoed, J. A. Stegeman, A. G. M. Tielens, R. Jorritsma^*

^*Corresponding author for this work

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

An in vitro model was used to investigate effects of beta-hydroxybutyrate and isoproterenol (beta-adrenergic receptor agonist) on lipolysis in isolated adipocytes from late pregnant and recently calved dairy cows (n = 5) and cows with clinical ketosis (n =3). Incubation with 3.0 mmol/L beta-hydroxybutyrate reduced lipolysis in isolated adipocytes. This inhibitory effect was lower in the first lactation week (47% +/- 16%) compared with late pregnancy (71% +/- 6.5%). Incubation with 0.3 mu mol/L isoproterenol stimulated lipolysis in isolated adipocytes from periparturient dairy cows. Basal lipolysis resulted in non-esterified fatty acid to glycerol ratios in the incubation media of 2.0 +/- 0.23 in prepartum samples, 2.1 +/- 0.23 in the first lactation week and 2.2 +/- 0.09 in cows with clinical ketosis. beta-Hydroxybutyrate reduced lipolysis by 45% +/- 9.6% in isolated adipocytes from cows with clinical ketosis, indicating that impaired feedback of beta-hydroxybutyrate may not play a role in the disease etiology. (C) 2012 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	433-439
Number of pages	7
Journal	Research in Veterinary Science
Volume	94
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2012.11.009
Publication status	Published - Jun 2013

Keywords

Dairy cow
Adipocyte
beta-Hydroxybutyrate
Isoproterenol
Ketosis
ADIPOSE-TISSUE METABOLISM
VOLATILE FATTY-ACIDS
KETONE-BODIES
TRANSITION PERIOD
RAT ADIPOCYTES
LACTATION
MOBILIZATION
EXPRESSION
CATTLE
DRY

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van der Drift, S. G. A., Everts, R. R., Houweling, M., van Leengoed, L. A. M. G., Stegeman, J. A., Tielens, A. G. M., & Jorritsma, R. (2013). Effects of beta-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis. Research in Veterinary Science, 94(3), 433-439. https://doi.org/10.1016/j.rvsc.2012.11.009

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title = "Effects of beta-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis",

abstract = "An in vitro model was used to investigate effects of beta-hydroxybutyrate and isoproterenol (beta-adrenergic receptor agonist) on lipolysis in isolated adipocytes from late pregnant and recently calved dairy cows (n = 5) and cows with clinical ketosis (n =3). Incubation with 3.0 mmol/L beta-hydroxybutyrate reduced lipolysis in isolated adipocytes. This inhibitory effect was lower in the first lactation week (47% +/- 16%) compared with late pregnancy (71% +/- 6.5%). Incubation with 0.3 mu mol/L isoproterenol stimulated lipolysis in isolated adipocytes from periparturient dairy cows. Basal lipolysis resulted in non-esterified fatty acid to glycerol ratios in the incubation media of 2.0 +/- 0.23 in prepartum samples, 2.1 +/- 0.23 in the first lactation week and 2.2 +/- 0.09 in cows with clinical ketosis. beta-Hydroxybutyrate reduced lipolysis by 45% +/- 9.6% in isolated adipocytes from cows with clinical ketosis, indicating that impaired feedback of beta-hydroxybutyrate may not play a role in the disease etiology. (C) 2012 Elsevier Ltd. All rights reserved.",

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author = "{van der Drift}, {S. G. A.} and Everts, {R. R.} and M. Houweling and {van Leengoed}, {L. A. M. G.} and Stegeman, {J. A.} and Tielens, {A. G. M.} and R. Jorritsma",

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AU - van der Drift, S. G. A.

AU - Everts, R. R.

AU - Houweling, M.

AU - van Leengoed, L. A. M. G.

AU - Stegeman, J. A.

AU - Tielens, A. G. M.

AU - Jorritsma, R.

PY - 2013/6

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KW - Dairy cow

KW - Adipocyte

KW - beta-Hydroxybutyrate

KW - Isoproterenol

KW - Ketosis

KW - ADIPOSE-TISSUE METABOLISM

KW - VOLATILE FATTY-ACIDS

KW - KETONE-BODIES

KW - TRANSITION PERIOD

KW - RAT ADIPOCYTES

KW - LACTATION

KW - MOBILIZATION

KW - EXPRESSION

KW - CATTLE

KW - DRY

U2 - 10.1016/j.rvsc.2012.11.009

DO - 10.1016/j.rvsc.2012.11.009

M3 - Article

SN - 0034-5288

VL - 94

SP - 433

EP - 439

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 3

ER -

Effects of beta-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis

Abstract

Keywords

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